A Toolkit for Rapid Modular Construction of Biological Circuits in Mammalian Cells

Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 10.1021/acssynbio.9b00322. Epub 2019 Nov 5. PubMed Article

These plasmids are not included in The Mammalian Toolkit. To order the Mammalian Toolkit, please see the following kit page.

Plasmids from Article

ID	Plasmid	Purpose
123952	MTK3_001	Encodes nuclear localized PHR domain of CRY2 fused to activating domain of p65 as Type 3 part to be used in the MTK system
123953	MTK3b_023	Encodes human RelA as a Type 3b part to be used in the MTK system
123954	MTK3b_025	Encodes synTF fused to VP16 activation domain as a Type 3b part to be used in the MTK system
123955	MTK3b_026	Encodes synTF fused to VP64 activation domain as a Type 3b part to be used in the MTK system
123956	MTK4a_009	Encodes a nuclear localized EBFP2 (T2A) coexpression cassette as a Type 4a part to be used in the MTK system
123957	MTK234_009	Encodes the spCas9 pC120 gRNA expression cassette as a type 234 part to be used in the MTK system
123958	MTK234_011	Encodes the spCas9 pPLMP1 gRNA expression cassette as a type 234 part to be used in the MTK system
123959	MTK234_054	Encodes the saCas9 CXCR1 (site 3) gRNA expression cassette as a type 234 part to be used in the MTK system
123960	MTK0_002	Encodes the piggyBac GFP dropout destination vector with the 2xHS4 insulator as a type 0 part to be used in the MTK system
123961	MTK0_004	Encodes the Cas9 cROSA26 homology destination with Blasticidin resistance cassette GFP dropout destination vector as a type 0 part to be used in the MTK system
123964	MTK0_009	Encodes PhiC31 landing pad as a type 0 part to be used in the MTK system
123965	MTK0_010	Encodes BxBI landing pad as a type 0 part to be used in the MTK system
123966	MTK0_011	Encodes the hROSA26 PhiC31 recognition site with Hygromycin resistance and mRuby2 landing pad with GFP dropout destination vector as a type 0 part to be used in the MTK system
123967	MTK0_012	Encodes the hROSA26 BxBI recognition site with Hygromycin resistance and mRuby2 landing pad with GFP dropout destination vector as a type 0 part to be used in the MTK system
123968	MTK0_028	Encodes the cROSA26 homology landing pad with GFP dropout destination vector as a type 0 part to be used in the MTK system
123969	MTK0_030	Encodes the phiC31-hygromycin-mRuby2PEST landing pad as a type 0 part to be used in the MTK system
123970	MTK0_031	Encodes the BxBI-hygromycin-mRuby2PEST landing pad as a type 0 part to be used in the MTK system
123971	MTK0_033	Encodes the cROSA26 PhiC31 landing pad as a type 0 part to be used in the MTK system
123972	MTK0_034	Encodes the hROSA26 PhiC31 landing pad as a type 0 part to be used in the MTK system
123973	MTK0_036	Encodes the cROSA26 BxBI landing pad as a type 0 part to be used in the MTK system
123974	MTK0_037	Encodes the hROSA26 BxBI landing pad as a type 0 part to be used in the MTK system
123975	MTK0_045	Encodes the cROSA26 destination with hygyomycin resistance cassette as a type 0 part to be used in the MTK system
123976	MTK0_046	Encodes the Cas9 Thal5.10-2_hg18 homology destination with Hygromycin resistance cassette GFP dropout destination vector as a type 0 part to be used in the MTK systemh
123977	MTK0_047	Encodes the Cas9 hCLYBL homology destination with Hygromycin resistance cassette GFP dropout destination vector as a type 0 part to be used in the MTK system
123978	MTK0_052	Encodes the PhiC31 landing pad on hThal5.10-2-hg18 with hygromycin resistance cassette coexpressed with mRuby2 as a type 0 part to be used in the MTK system
123980	MTK0_054	Encodes the PhiC31 landing pad on hCLYBL with hygromycin resistance cassette coexpressed with mRuby2 as a type 0 part to be used in the MTK system
123982	MTK234_057	Encodes the spCas9 Safetargeting Chr20 gRNA expression cassette as a type 234 part to be used in the MTK system
123984	MTK234_058	Encodes the spCas9 Safetargeting Chr11 gRNA expression cassette as a type 234 part to be used in the MTK system
123988	MTK234_012	Encodes the spCas9 sgRNA1 hROSA26 (site 1) gRNA expression cassette as a type 234 part to be used in the MTK system
123989	MTK234_013	Encodes the spCas9 sgRNA1 hROSA26 (site 2) gRNA expression cassette as a type 234 part to be used in the MTK system
123990	MTK234_014	Encodes the spCas9 sgRNA1 hROSA26 (site 3) gRNA expression cassette as a type 234 part to be used in the MTK system
123991	MTK234_015	Encodes the spCas9 sgRNA1 hROSA26 (site 4) gRNA expression cassette as a type 234 part to be used in the MTK system
123992	MTK234_005	Encodes the spCas9 sgRNA1 cROSA26 (homology arm 1) gRNA expression cassette as a type 234 part to be used in the MTK system
123993	MTK234_006	Encodes the spCas9 sgRNA1 cROSA26 (homology arm 2) gRNA expression cassette as a type 234 part to be used in the MTK system
123994	MTK234_007	Encodes the spCas9 sgRNA1 cROSA26 (homology arm 3) gRNA expression cassette as a type 234 part to be used in the MTK system
123995	MTK234_032	Encodes the spCas9 sgRNA1 hAAVS1 (site 1) gRNA expression cassette as a type 234 part to be used in the MTK system
123996	MTK234_033	Encodes the spCas9 sgRNA1 hAAVS1 (site 2) gRNA expression cassette as a type 234 part to be used in the MTK system
123997	MTK234_034	Encodes the spCas9 sgRNA1 hAAVS1 (site 3) gRNA expression cassette as a type 234 part to be used in the MTK system
123998	JPF0507	Encodes pPGK1 driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
123999	JPF0508	Encodes pCAG driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
124000	JPF0509	Encodes pPGK1 driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
124001	JPF0510	Encodes pEF1a driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
124002	JPF0511	Encodes pHUBC driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
124003	JPF0512	Encodes promoter-less expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
124004	JPF0513	Encodes pCMV-A driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
124005	JPF0514	Encodes pCMV-B driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
124006	JPF0515	Encodes pCMV-C driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
124007	JPF0516	Encodes pCMV-D driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
124008	JPF0517	Encodes pCMV-E driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
124009	JPF0518	Encodes pCMV-F driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
124010	JPF0519	Encodes pCMV-G driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
124011	JPF0520	Encodes pCMV driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
124012	JPF0521	Encodes crippled pPGK1 driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
124013	JPF0522	Encodes crippled pEF1a driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
124014	JPF0523	Encodes crippled pSFFV driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
124015	ARB287a	Encodes pEF1a driving expression of mAzamiGreen with a BgpPA 3' UTR and a mScarlet normalization in a PiggyBac destination vector
124016	ARB287b	Encodes pEF1a driving expression of mAzamiGreen with a SV40 3' UTR and a mScarlet normalization in a PiggyBac destination vector
124017	ARB287c	Encodes pEF1a driving expression of mAzamiGreen with a RglPA 3' UTR and a mScarlet normalization in a PiggyBac destination vector
124018	ARB287d	Encodes pEF1a driving expression of mAzamiGreen with a spacer 3' UTR and a mScarlet normalization in a PiggyBac destination vector
124019	ARB287e	Encodes pEF1a driving expression of mAzamiGreen with a multicistronic spacer 3' UTR and a mScarlet normalization in a PiggyBac destination vector
124020	JPF0419d	Encodes pCAG driving expression of multicistronic Lyn-tagged iRFP713, cytoplasmic mAzamiGreen, mCerulean-tethered p38 KTR, and Histone 2B fused to mScarlet in a PiggyBac destination vector
124021	JPF0419v	Encodes pCAG driving expression of multicistronic Lyn-tagged iRFP713, cytoplasmic mAzamiGreen, mCerulean-tethered p38 KTR, and Histone 2B fused to mScarlet in a PiggyBac destination vector
124022	JPF0446	BxBI Destination vector with H2B::GFP expressed from CAG promoter
124023	ARB340	Encodes TU with connectors LS R1 and an A4 insulator, and pEF1a expressing nuclear iRFP713
124024	ARB341	Encodes TU with connectors LS R1 and an A4 insulator, and pEF1a expressing nuclear tagBFP
124025	ARB342	Encodes TU with connectors LS R1 and an A4 insulator, and pEF1a expressing nuclear mAzamiGreen
124026	ARB343	Encodes TU with connectors LS R1 and an A4 insulator, and pEF1a expressing nuclear mRuby2
124027	ARB344	Encodes TU with connectors L1 R2 and an B1 insulator, and pCMV expressing cytoplasmic iRFP713
124028	ARB345	Encodes TU with connectors L1 R2 and an B1 insulator, and pCMV expressing cytoplasmic tagBFP
124029	ARB346	Encodes TU with connectors L1 R2 and an B1 insulator, and pCMV expressing cytoplasmic mAzamiGreen
124030	ARB347	Encodes TU with connectors L1 R2 and an B1 insulator, and pCMV expressing cytoplasmic mRuby2
124031	ARB348	Encodes TU with connectors L2 R3 and an C3 insulator, and pCAG expressing membrane-tagged iRFP713
124032	ARB349	Encodes TU with connectors L2 R3 and an C3 insulator, and pCAG expressing membrane-tagged tagBFP
124033	ARB350	Encodes TU with connectors L2 R3 and an C3 insulator, and pCAG expressing membrane-tagged mAzamiGreen
124034	ARB351	Encodes TU with connectors L2 R3 and an C3 insulator, and pCAG expressing membrane-tagged mRuby2
124035	JPF0464	Encodes pEF1a driving expression of nuclear iRFP713, and mu6 expression of a human non-targeting sgRNA in a 2nd gen. Lenti Virus destination vector
124036	JPF0465	Encodes pEF1a driving expression of nuclear iRFP713, and mu6 expression of a mScarlet-targeting sgRNA in a 2nd gen. Lenti Virus destination vector
124037	JPF0466	Encodes pEF1a driving expression of nuclear iRFP713, and mu6 expression of a tagBFP-targeting sgRNA in a 2nd gen. Lenti Virus destination vector
124038	JPF0468	Encodes pEF1a driving expression of nuclear iRFP713, and mu6 expression of tagBFP- and mScarlet-targeting sgRNAs in a 2nd gen. Lenti Virus destination vector
124039	JPF0454	Encodes pPGK1 driving expression of spCas9 P2A mAzamiGreen in a 2nd gen. Lenti Virus destination vector
124040	JPF0455	Encodes pPGK1 driving expression of cytoplasmic tagBFP in a 2nd gen. Lenti Virus destination vector
124041	JPF0456	Encodes pPGK1 driving expression of mScarlet in a 2nd gen. Lenti Virus destination vector
124042	JPF0535a	Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing spdCas9 fused to tagBFP P2A tagBFP, and an sgRNA targeting pTRE expressing mAzamiGreen in a PiggyBac destination vector
124048	ARB365	Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to mRuby2 P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vector
124049	ARB366	Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to KRAB domain P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vector
124050	ARB367	Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to VPR domain P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vector
124054	MTK3_028	Encodes c120 sg RNA guide cleaved by ribozyme
124055	MTK234_047	Encodes the spCas9 mScarlet (site 2) gRNA expression cassette as a type 234 part to be used in the MTK system
124056	MTK234_048	Encodes the spCas9 mScarlet (site 3) gRNA expression cassette as a type 234 part to be used in the MTK system
124057	MTK234_049	Encodes the spCas9 mScarlet (site 4) gRNA expression cassette as a type 234 part to be used in the MTK system
124058	MTK6_016	Encodes the extended Woodchuck Hepatitis Virus (WHP) Posttranscriptional Regulatory Element (WPRE) to be used in the MTK system