Mapping the energetic and allosteric landscapes of protein binding domains.
Faure AJ, Domingo J, Schmiedel JM, Hidalgo-Carcedo C, Diss G, Lehner B
Nature. 2022 Apr;604(7904):175-183. doi: 10.1038/s41586-022-04586-4. Epub 2022 Apr 6.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 183619 | pGJJ001 | bindingPCA plasmid - Contains a barcode landing pad and two fragments of the murine methotrexate-resistant DHFR under the expression of two CYC promoters. |
| 183621 | pGJJ045 | abundancePCA plasmid - Contains a barcode landing pad and two fragments of the murine methotrexate-resistant DHFR, the expression of one driven by the CYC promoter and the other by the GPD promoter. |
| 183622 | pGJJ055 | ddPCA mutagenesis plasmid - Efficient mutant library cloning plasmid suitable for oligo synthesis, error prone PCR or nicking mutagenesis strategies. |