Mapping the energetic and allosteric landscapes of protein binding domains.

Faure AJ, Domingo J, Schmiedel JM, Hidalgo-Carcedo C, Diss G, Lehner B
Nature. 2022 Apr;604(7904):175-183. doi: 10.1038/s41586-022-04586-4. Epub 2022 Apr 6. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID	Plasmid	Purpose
183619	pGJJ001	bindingPCA plasmid - Contains a barcode landing pad and two fragments of the murine methotrexate-resistant DHFR under the expression of two CYC promoters.
183621	pGJJ045	abundancePCA plasmid - Contains a barcode landing pad and two fragments of the murine methotrexate-resistant DHFR, the expression of one driven by the CYC promoter and the other by the GPD promoter.
183622	pGJJ055	ddPCA mutagenesis plasmid - Efficient mutant library cloning plasmid suitable for oligo synthesis, error prone PCR or nicking mutagenesis strategies.