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Sperm membrane proteins DCST1 and DCST2 are required for sperm-egg interaction in mice and fish.

Noda T, Blaha A, Fujihara Y, Gert KR, Emori C, Deneke VE, Oura S, Panser K, Lu Y, Berent S, Kodani M, Cabrera-Quio LE, Pauli A, Ikawa M
Commun Biol. 2022 Apr 7;5(1):332. doi: 10.1038/s42003-022-03289-w. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
183540pClgn1.1_Dcst1-3xHAExpress Dcst1-3xHA under the mouse Clgn promoter.
183541pClgn1.1_Dcst2-3xHAExpress Dcst2-3xHA under the mouse Clgn promoter.
183542pCAG-Dcst1-3xFLAGExpression vector of mouse DC-STAMP domain containing 1 (Dcst1) tagged with 3xFLAG at C-terminus.
183543pCAG-Dcst2-3xHAExpression vector of mouse DC-STAMP domain containing 2 (Dcst2) tagged with 3xHA at C-terminus.
183544pCAG-Izumo1-1D4Expression vector of mouse izumo sperm-egg fusion 1 (Izumo1) tagged with 1D4 at C-terminus.

Antibodies from Article