High-throughput strategy for identification of Mycobacterium tuberculosis membrane protein expression conditions using folding reporter GFP.
Grave K, Bennett MD, Hogbom M
Protein Expr Purif. 2022 Oct;198:106132. doi: 10.1016/j.pep.2022.106132. Epub 2022 Jun 21.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 183509 | pYUBcLIC-GFP | The plasmid contains LIC cassette upstream of folding reporter GFP and TEV-cleavable 10x histidine purification tag. Compatible with T7 promoter-based expression system in mycobacteria. |
| 183514 | pETcLIC-GFP | The plasmid contains LIC cassette upstream of folding reporter GFP and TEV-cleavable 10x histidine purification tag. Compatible with T7 promoter-based expression system in E. coli |