Highly specific and efficient CRISPR/Cas9-catalyzed homology-directed repair in Drosophila.

Gratz SJ, Ukken FP, Rubinstein CD, Thiede G, Donohue LK, Cummings AM, O'Connor-Giles KM
Genetics. 2014 Apr;196(4):961-71. doi: 10.1534/genetics.113.160713. Epub 2014 Jan 29. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID	Plasmid	Purpose
46294	pBS-Hsp70-Cas9	A codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter.
51019	pDsRed-attP	Vector for generating dsDNA donors for homology-directed repair to replace genes or other genomic sequence with an attP docking site. Contains the visible marker 3xP3-DsRed. As known as pHD-DsRed-attP
51434	pHD-DsRed	Vector for generating dsDNA donors for homology-directed repair. Contains the visible marker 3xP3-DsRed.