AKAP79 enables calcineurin to directly suppress protein kinase A activity.
Church TW, Tewatia P, Hannan S, Antunes J, Eriksson O, Smart TG, Hellgren Kotaleski J, Gold MG
Elife. 2021 Oct 6;10. pii: 68164. doi: 10.7554/eLife.68164.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 183454 | pFUGW-shRIIα | sgRNA targeting rat PKA-RIIα subunits |
| 183455 | pFUGW-scrambled | This control vector contains a scrambled version of the targeting sequence used in the pFUGW-shRIIα construct |
| 183456 | pFUGW-shRIIα-RIIαWT-IRES-EGFP | Replacement of endogenous rat PKA-RIIα subunits with wild-type RIIα |
| 183457 | pFUGW-shRIIα-RIIαS97A-IRES-EGFP | Replacement of endogenous rat PKA-RIIα subunits with the RIIα S97A variant |
| 183458 | pFUGW-shRIIα-RIIαS97E-IRES-EGFP | Replacement of endogenous rat PKA-RIIα subunits with the RIIα S97E variant |