The HASTER lncRNA promoter is a cis-acting transcriptional stabilizer of HNF1A.
Beucher A, Miguel-Escalada I, Balboa D, De Vas MG, Maestro MA, Garcia-Hurtado J, Bernal A, Gonzalez-Franco R, Vargiu P, Heyn H, Ravassard P, Ortega S, Ferrer J
Nat Cell Biol. 2022 Oct 6. pii: 10.1038/s41556-022-00996-8. doi: 10.1038/s41556-022-00996-8.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 183231 | pLV-hIP-dCas9-BSD | Lentiviral vectors with the human insulin promoter driving expression of dCas9, in addition to a U6-driven sgRNA |
| 183232 | pLenti-CMVtight-HNF1A-FLAG-Hygro | Lentiviral vector; Tet-on advanced system driving the expression of tagged HNF1A |
| 183233 | pLV-rtTA-zeo | Lentiviral vector expressing the rtTa gene |
| 183234 | pcDNA3.1-flag-UTR | Mammalian expression empty backbone |
| 183235 | pcDNA3.1-GFP-Flag-UTR | To express FLAG tagged eGFP |
| 183236 | pcDNA3.1-GFP-FUS-IDR | Expression of eGFP fused to a intrinsically disordered regions of the FUS protein |
| 183237 | pcDNA3.1-HNF1A-WT-Flag-UTR | Expression of FLAG tagged HNF1A |
| 183238 | pcDNA3.1-HNF1A-delta-IDR2 | Expression of FLAG tagged HNF1A with deletion of a intrinsically disordered regions (AA545-573) |
| 183239 | pcDNA3.1-HNF1B-WT-Flag-UTR | Expression of FLAG tagged HNF1B |
| 183240 | pcDNA3.1-HNF1B-FUS-IDR | Expression of HNF1B fused to a intrinsically disordered regions of the FUS protein |