Generation of dual-gRNA library for combinatorial CRISPR screening of synthetic lethal gene pairs.
Tang S, Wu X, Liu J, Zhang Q, Wang X, Shao S, Gokbag B, Fan K, Liu X, Li F, Cheng L, Li L
STAR Protoc. 2022 Aug 24;3(3):101556. doi: 10.1016/j.xpro.2022.101556. eCollection 2022 Sep 16.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 183192 | lentiCRISPR_DKO | Plasmid with Cas9, two U6 promoters and two gRNA scaffolds that allows for inserting two gRNAs for combinatorial CRISPR Screen or double-knock-out (DKO) screen |
| 183193 | LentiGuide_DKO | Plasmid with two U6 promoters and two gRNA scaffolds that allows for inserting two gRNAs for combinatorial CRISPR Screen or double-knock-out (DKO) screen |