Localized Proteolysis for the Construction of Intracellular Asymmetry in Escherichia coli.
Hong JC, Fan HC, Yang PJ, Lin DW, Wu HC, Huang HC
ACS Synth Biol. 2021 Aug 20;10(8):1830-1836. doi: 10.1021/acssynbio.1c00200. Epub 2021 Aug 10.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 183189 | TDGS50 | TEV protease expressed under J23106 promoter; DivIVAsfGFP-TEV protease recognition site-M0050 expressed under J23116 promoter |
| 183190 | NSSCP | mRFP-PopZ expressed under J23116 promoter; split N terminal of TEV protease-SpmXΔC expressed under Tac promoter; SpmXΔC-split C terminal of TEV protease expressed under Tac promoter; DivIVA-sfGFP-TEV |