All-in-one: A Robust Fluorescent Fusion Protein Vector Toolbox for Protein Localization and BiFC Analyses in Plants.
Han J, Ma K, Li H, Su J, Zhou L, Tang J, Zhang S, Hou Y, Chen L, Liu YG, Zhu Q
Plant Biotechnol J. 2022 Feb 18. doi: 10.1111/pbi.13790.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 183151 | pYL322d1/N-eGFP | Plant transient expression vector containing eGFP tag. Unique nucleotide sequences Uc and Ud for cloning at N terminus of eGFP. |
| 183152 | pYL322d1/eGFP-C | Plant transient expression vector containing eGFP tag. Unique nucleotide sequences Uc and Ud for cloning at C terminus of eGFP. |
| 183153 | pYL322d1/N-Vn | Bimolecular fluorescence complementation (BiFC) vector for transient expression of N-truncated Venus in plants. Unique nucleotide sequences Uc and Ud for cloning at N terminus of Vn. |
| 183154 | pYL322d1/Vn-C | Bimolecular fluorescence complementation (BiFC) vector for transient expression of N-truncated Venus in plants. Unique nucleotide sequences Uc and Ud for cloning at C terminus of Vn. |
| 183155 | pUC19/N-eCFP | Plant transient expression vector containing eCFP tag. Unique nucleotide sequences Uc and Ud for cloning at N terminus of eCFP |
| 183156 | pUC19/eCFP-C | Plant transient expression vector containing eCFP tag. Unique nucleotide sequences Uc and Ud for cloning at C terminus of eCFP |
| 183157 | pUC19/N-Vc | Bimolecular fluorescence complementation (BiFC) vector for transient expression of C-truncated Venus in plants. Unique nucleotide sequences Uc and Ud for cloning at N terminus of Vc. |
| 183158 | pUC19/Vc-C | Bimolecular fluorescence complementation (BiFC) vector for transient expression of C-truncated Venus in plants. Unique nucleotide sequences Uc and Ud for cloning at C terminus of Vc. |
| 183159 | pUC19/N-mChe | Plant transient expression vector containing mCherry tag. Unique nucleotide sequences Uc and Ud for cloning at N terminus of mCherry. |
| 183160 | pUC19/mChe-C | Plant transient expression vector containing mCherry tag. Unique nucleotide sequences Uc and Ud for cloning at C terminus of mCherry. |
| 183161 | pUC19/mChe | Construct for transient expression of cell nucleus-, cytoplasm-targeted mCherry in plants. |
| 183162 | pUC19/NLS-mChe | Construct for transient expression of cell nucleus-targeted mCherry in plants. |
| 183163 | pUC19/ER-mChe | Construct for transient expression of endoplasmic reticulum-targeted mCherry in plants. |
| 183164 | pUC19/Pt-mChe | Construct for transient expression of plastids-targeted mCherry in plants. |
| 183165 | pUC19/Tp-mChe | Construct for transient expression of tonoplast-targeted mCherry in plants. |
| 183166 | pUC19/CF-mChe | Construct for transient expression of cytoplasmic foci-targeted mCherry in plants. |
| 183167 | pUC19/Mito-mChe | Construct for transient expression of mitochondrion-targeted mCherry in plants. |
| 183168 | pUC19/mChe-PM | Construct for transient expression of plasma membrane-targeted mCherry in plants. |
| 183169 | pUC19/mChe-Golgi | Construct for transient expression of Golgi apparatus-targeted mCherry in plants. |
| 183170 | pUC19/mChe-Px | Construct for transient expression of peroxisomes-targeted mCherry in plants. |
| 183171 | pUC19/mChe-Cs | Construct for transient expression of cytoskeleton-targeted mCherry in plants. |
| 183172 | pUC19/Pd-mChe | Construct for transient expression of plasmodesmata-targeted mCherry in plants. |
| 183173 | pYL1300UaUf | Plant binary vector with ccdB flanked by Ua and Uf unique nucleotide sequences. |