Construction of intracellular asymmetry and asymmetric division in Escherichia coli.
Lin DW, Liu Y, Lee YQ, Yang PJ, Ho CT, Hong JC, Hsiao JC, Liao DC, Liang AJ, Hung TC, Chen YC, Tu HL, Hsu CP, Huang HC
Nat Commun. 2021 Feb 9;12(1):888. doi: 10.1038/s41467-021-21135-1.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
183187 | pNSSCPG | PACE-evolved split N (core) terminal of T7 RNA polymerase-SpmXΔC and SpmXΔC-PACE-evolved split C (sigma) terminal of T7 RNA polymerase both expressed under Tac promoter; sfGFP-LAA expressed under T7 p |
183188 | pNSSCP | PACE-evolved split N (core) terminal of T7 RNA polymerase-SpmXΔC and SpmXΔC-PACE-evolved split C (sigma) terminal of T7 RNA polymerase both expressed under Tac promoter; DivIVA-sfGFP expressed under T |