Scalable biological signal recording in mammalian cells using Cas12a base editors.
Kempton HR, Love KS, Guo LY, Qi LS
Nat Chem Biol. 2022 May 30. pii: 10.1038/s41589-022-01034-2. doi: 10.1038/s41589-022-01034-2.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 183098 | pSLQ11353 | pHR-CAG-sfGFP* ABE_3-WPRE |
| 183203 | pSLQ11440 | pHR-TRE3G-ABE8e-hyperdLbCas12a-NLS-P2A-mCherry-EFS-Puro-WPRE |
| 183204 | pSLQ11438 | pHR-EF1a-ABE8e-hyperdLbCas12a-NLS-mCherry-P2A-Puro-WPRE |
| 183205 | pSLQ11429 | pHR-R(NFKB-BFP-Triplex-2X crGFP*ABE3-SV40pA)-EFS-Hygro-T2A-rtTA-WPRE |
| 183206 | pSLQ11453 | PB-NFKB-BFP-Triplex-crAAVS1_4-SV40pA |
| 183207 | pSLQ11350 | PB-TRE3G-BFP-Triplex-crGFP*ABE3-SV40pA |
| 183623 | pSLQ11465 | PB-10X IRF-minCMV-BFP-Triplex-crAAVS1_8-SV40pA |
| 183624 | pSLQ11474 | PB-STAT6RE-minCMV-BFP-Triplex-crAAVS1_6-SV40pA |
| 183625 | pSLQ11434 | pHR-R(HRE-minCMV-BFP-Triplex-2X crGFP*ABE3-SV40pA)-EFS-Hygro-T2A-rtTA-WPRE |
| 183626 | pSLQ11435 | pHR-R(CRE-minCMV-BFP-Triplex-2X crGFP*ABE3-SV40pA)-EFS-Hygro-T2A-rtTA-WPRE |
| 183627 | pSLQ11443 | pHR-R(NFAT-minCMV-BFP-Triplex-2X crGFP*ABE3-SV40pA)-EFS-Hygro-T2A-rtTA-WPRE |
| 183628 | pSLQ11444 | pHR-R(AP1-minCMV-BFP-Triplex-2X crGFP*ABE3-SV40pA)-EFS-Hygro-T2A-rtTA-WPRE |
| 183629 | pSLQ11445 | pHR-R(ARE-minCMV-BFP-Triplex-2X crGFP*ABE3-SV40pA)-EFS-Hygro-T2A-rtTA-WPRE |