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A Well-Characterized Polycistronic-Like Gene Expression System in Yeast.

Mukherjee M, Wang ZQ
Biotechnol Bioeng. 2022 Sep 27. doi: 10.1002/bit.28247. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
183082pYTK001_E2Ab2A peptide E2A cloned in yeast MoClo entry vector (pYTK001) for use in bi-cistronic and as the first 2A peptide in tri-cistronic construct assembly
183083pYTK001_O2Ab2A peptide O2A cloned in yeast MoClo entry vector (pYTK001) for use in bi-cistronic and as the first 2A peptide in tri-cistronic construct assembly
183084pYTK001_P2Ab2A peptide P2A cloned in yeast MoClo entry vector (pYTK001) for use in bi-cistronic and as the first 2A peptide in tri-cistronic construct assembly
183085pYTK001_E2At(2nd)2A peptide E2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in tri-cistronic and third 2A peptide in quad-cistronic construct assembly
183086pYTK001_O2At(2nd)2A peptide O2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in tri-cistronic and third 2A peptide in quad-cistronic construct assembly
183087pYTK001_P2At(2nd)2A peptide P2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in tri-cistronic and third 2A peptide in quad-cistronic construct assembly
183088pYTK001_E2Aq(1st)2A peptide E2A cloned in yeast MoClo entry vector (pYTK001) for use as the first 2A peptide in quad-cistronic construct assembly
183089pYTK001_P2Aq(2nd)2A peptide P2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in quad-cistronic construct assembly
190911pYTK001_E2Aq(2nd)2A peptide E2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in quad-cistronic construct assembly
190912pYTK001_P2Aq(1st)2A peptide P2A cloned in yeast MoClo entry vector (pYTK001) for use as the first 2A peptide in quad-cistronic construct assembly
190917pYTK001_O2Aq(1st)2A peptide O2A cloned in yeast MoClo entry vector (pYTK001) for use as the first 2A peptide in quad-cistronic construct assembly
190918pYTK001_O2Aq(2nd)2A peptide O2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in quad-cistronic construct assembly

Antibodies from Article