A naturally DNase-free CRISPR-Cas12c enzyme silences gene expression.

Huang CJ, Adler BA, Doudna JA
Mol Cell. 2022 Jun 2;82(11):2148-2160.e4. doi: 10.1016/j.molcel.2022.04.020. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
183069	pCJH002_10xHis-MBP-TEVcs-Cas12c(4)_Amp	Bacterial protein expression plasmid of wild-type Cas12c_4. This is a R965H version of the Cas12c in Harrington et al., 2020.
183070	pCJH009_proD_Cas12c-sgRNA-GFP_CAM	Plasmid expressing Cas12c sgRNA targeting GFP (1) for in vivo interference assay in bacteria.
183071	pCJH019_SC101_pLAC_Cas12c(4)_Kan	Wild-type Cas12c_4 in a plasmid containing a SC101 origin and lac promoter for in vivo interference assay.
183072	pCJH021_SC101_pLAC_Cas12c2_Kan	Wild-type Cas12c2 (Yan et al., 2019) in a plasmid containing a SC101 origin and lac promoter for in vivo interference assay.
183073	pCJH026_proD_Cas12c-multiplexing_CAM	Plasmid expressing a Cas12c sgRNA targeting GFP and another sgRNA targeting RFP in one transcript for testing multiplexed repression
183074	pCJH027_SC101_pLAC_LbCas12a_Kan	Wild-type LbCas12a (Chen et al., 2018) in a plasmid containing a SC101 origin and lac promoter for in vivo interference assay.
183075	pCJH031_proD_LbCas12a-gRNA-GFP_CAM	Plasmid expressing Cas12a guide RNA targeting GFP (1) for in vivo interference assay in bacteria.
183076	pCJH059_proD_Cas12c_DualRNA-GFP_CAM	Plasmid expressing Cas12c tracrRNA and a GFP-targeting pre-crRNA separately.
183077	pCJH065_proD_Cas12c_DualRNA-GFP+HDVrz_CAM	Plasmid expressing Cas12c tracrRNA and GFP-targeting crRNA separately, with the crRNA ending with a HDV ribozyme sequence.
183221	Cas12c-sgRNA_BbsI_CAM	Cas12c sgRNA containing BbsI restriction recognition sites in spacer sequence for Golden Gate Assembly
183222	LbCas12a-gRNA_BbsI_CAM	LbCas12a gRNA containing BbsI restriction recognition sites in spacer sequence for Golden Gate Assembly