Identification of acetic acid sensitive strains through biosensor-based screening of a Saccharomyces cerevisiae CRISPRi library.
Mormino M, Lenitz I, Siewers V, Nygard Y
Microb Cell Fact. 2022 Oct 15;21(1):214. doi: 10.1186/s12934-022-01938-7.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 183062 | pMM4_14 | Plasmid expressing a biosensor for acetic acid: BM3R1-Haa1-mTurquoise2 under RET2p and mCherry under HREsYGP1p with flanks for genome integration into HO locus |
| 183063 | pMM4_14L | Plasmid expressing a biosensor for acetic acid and pHluorin: BM3R1-Haa1-mTurquoise2 under RET2p, mCherry under HREsYGP1p and sfpHluorin under TDH3p with flanks for genome integration into HO locus |
| 207623 | IL1_1 | Low copy plasmid expressing mTurquoise2 under the TDH3p promoter |