Development of an Haa1-based biosensor for acetic acid sensing in Saccharomyces cerevisiae.
Mormino M, Siewers V, Nygard Y
FEMS Yeast Res. 2021 Sep 11;21(6). pii: 6363685. doi: 10.1093/femsyr/foab049.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 183057 | pMM2_4 | HAA1-mTurquoise2 under HAA1p and mRuby2 under YGP1p with flanks for genome integration into HO locus |
| 183058 | pMM2_9 | Biosensor for acetic acid: BM3R1-Haa1-mTurquoise2 under HAA1p and mCherry under BM3R1ENO1cp with flanks for genome integration in toHO locus |
| 183059 | pMM2_10 | Control for biosensor in pMM2_9: HAA1-mTurquoise2 under HAA1p and mCherry under HREsYGP1p with flanks for genome integration into HO locus |
| 183060 | pMM2_11 | Control for biosensor in pMM2_9: HAA1-mTurquoise2 under HAA1p and mCherry under HREsENO1cp with flanks for genome integration into HO locus |
| 183061 | pMM2_12 | Control for biosensor in pMM2_9: BM3R1-HAA1-mTurquoise2 under HAA1p and mCherry under HREsYGP1p with flanks for genome integration into HO locus |