Multiplexed bioluminescence microscopy via phasor analysis.

Yao Z, Brennan CK, Scipioni L, Chen H, Ng KK, Tedeschi G, Parag-Sharma K, Amelio AL, Gratton E, Digman MA, Prescher JA
Nat Methods. 2022 Jul;19(7):893-898. doi: 10.1038/s41592-022-01529-9. Epub 2022 Jun 23. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
183049	pLenti_CMV: Flag-Antares_SV40:PuroR	Expresses FLAG-tagged Antares in mammalian cells.
183048	pLenti_CMV: Flag-Akaluc_SV40:PuroR	Expresses FLAG-tagged AkaLuc (Far-Red Firefly luciferase) in mammalian cells.
183047	pLenti_CMV:Flag-KaNLuc_SV40:PuroR	Expresses the FLAG-tagged KaNLuc (Far-Red LSSmKate2 LumiFluor) in mammalian cells.
183046	pLenti_CMV:Flag-dKeNLuc_SV40:PuroR	Expresses the FLAG-tagged dKeNLuc (Far-Red dKeima LumiFluor) in mammalian cells.
183045	pLenti_CMV:Flag-NLuc_SV40:PuroR	Expresses FLAG-tagged NanoLuciferase (NLuc-only) in mammalian cells.
183044	pLenti_CMV:Flag-LumiScarlet_SV40:PuroR	Expresses FLAG-tagged LumiScarlet in mammalian cells.
183042	pLenti_CMV:Flag-OeNL_SV40:PuroR	Expresses the FLAG-tagged OeNL (Orange Nanolantern) in mammalian cells.
183041	pLenti_CMV:Flag-YeNL_SV40:PuroR	Expresses the FLAG-tagged YeNL (Yellow Nanolantern) in mammalian cells.
183040	pLenti_CMV:Flag-GeNL_SV40:PuroR	Expresses the FLAG-tagged GeNL (Green Nanolantern) in mammalian cells.
183039	pLenti_CMV:Flag-CeNL_SV40:PuroR	Expresses the FLAG-tagged CeNL (Blue Nanolantern) in mammalian cells.