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Third-generation rabies viral vectors allow nontoxic retrograde targeting of projection neurons with greatly increased efficiency.

Jin L, Sullivan HA, Zhu M, Lea NE, Lavin TK, Fu X, Matsuyama M, Hou Y, Feng G, Wickersham IR
Cell Rep Methods. 2023 Nov 20;3(11):100644. doi: 10.1016/j.crmeth.2023.100644. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
182964pRV∆L-5CreThe 3rd generation of rabies vector expressing Cre recombinase
182965pRV∆L-5FlpoThe 3rd generation of rabies vector expressing Flpo recombinase
182966pRV∆L-5tTAThe 3rd generation of rabies vector expressing a tetracycline-controlled transactivator (tTA)
182967pB-TREtight-EGFPFor making cell lines for titering pRV∆L-5tTA virus
201831pB-CAG-FLEX-F14F15-BFP-(mCherry)'piggyBac transposon plasmid for making Cre and Flpo reporter cell lines using piggyBac transposase

Antibodies from Article