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Reconstitution of purified membrane protein dimers in lipid nanodiscs with defined stoichiometry and orientation using a split GFP tether.

Bruguera ES, Mahoney JP, Weis WI
J Biol Chem. 2022 Jan 21:101628. doi: 10.1016/j.jbc.2022.101628. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
182931FLAG-3C-sGFP1-10_pcDNA3.1(-)For expression of membrane protein with N-terminal FLAG tag and C-terminal split GFP1-10 fragment
182932FLAG-3C-sGFP11_pcDNA3.1(-)For expression of membrane protein with N-terminal FLAG tag and C-terminal split GFP11 fragment

Antibodies from Article