Augmin accumulation on long-lived microtubules drives amplification and kinetochore-directed growth.
David AF, Roudot P, Legant WR, Betzig E, Danuser G, Gerlich DW
J Cell Biol. 2019 Jul 1;218(7):2150-2168. doi: 10.1083/jcb.201805044. Epub 2019 May 21.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 182885 | Lenti_EGFP-P2A_truncHaus6_IRES_Blast | Transfer vector for production of lentivirus. Control plasmid for rescue experiment of HAUS6 depletion phenotype, containing a nonsense mutation and a truncation in HAUS6. |
| 182886 | Lenti_EGFP-P2A-HAUS6_IRES_Blast | Transfer vector for production of lentivirus. Expresses EGFP-P2A-HAUS6 (WT full length) |
| 182887 | Lenti_EGFP-P2A-siRNAresHAUS6_IRES_Blast | Transfer vector for production of lentivirus. Expresses EGFP-P2A-HAUS6 (siRNA resistant version) |