Trithorax monomethylates histone H3K4 and interacts directly with CBP to promote H3K27 acetylation and antagonize Polycomb silencing.
Tie F, Banerjee R, Saiakhova AR, Howard B, Monteith KE, Scacheri PC, Cosgrove MS, Harte PJ
Development. 2014 Mar;141(5):1129-39. doi: 10.1242/dev.102392.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 182839 | pGEX-2T-CBP(1-287) | CBP(1-287) was inserted into pGEX-2T with NdeI and NsiI sites. pGEX-2T-CBP(1-287) was used in the GST pull-down assay described in our paper (Tie et al. 2012 MCB, Tie et al 2014 Development) |
| 182844 | pGEX-2T-TRX-C751-E3616S | mutation E3616S (GAA/TCA), PCR product coding C-terminal residues 2976-3726 of TRX was inserted into modified pGEX-2T by Nde I-Nsi I sites. Expression in E. coli. by IPTG induction |
| 182845 | pGEX-2T-TRR-C421-G2304D | mutation G2304D (GGC/GAC), PCR product coding C-terminal residues 2011-2431 of TRR was inserted into modified pGEX-2T by Nde I-Nsi I sites |
| 182846 | pGEX-2T-TRR-C421-Y2404F | mutation Y2404F (TAC/TTC) in GST-TRR-C421 (TRR 2011-2431 aa). Expression in E. coli. by IPTG induction |