CBP-mediated acetylation of histone H3 lysine 27 antagonizes Drosophila Polycomb silencing.
Tie F, Banerjee R, Stratton CA, Prasad-Sinha J, Stepanik V, Zlobin A, Diaz MO, Scacheri PC, Harte PJ
Development. 2009 Sep;136(18):3131-41. doi: 10.1242/dev.037127.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 182840 | pMT-FLAG-CBPΔN | A 5.2 kb Drosophila CBP cDNA fragment (cut by Nhe I and Nsi I), encoding C-terminal residues 1476-3222, was inserted into the pMT-FLAG vector. Expression in S2 cells by CuSO4 induction. |
| 182841 | pMT-FLAG-CBPΔ(N+HAT) | An EcoRV fragment (encoding residues 1566-2142) was removed from pMT-FLAG-CBPΔN. Expression in S2 cells by CuSO4 induction. |
| 182842 | pAct-GST-H3 | A PCR product encoding GST-H3 (Drosophila histone H3) was was inserted into a modified pAct-5c vector by Sac I and Sal I sites. Expression in S2 cells. |
| 182843 | pAct-GST-H3(K27Q) | a K27Q point mutation (AAG/CAG) of Drosophila histone H3 was generated from pAct-GST-H3. Expression in S2 cells. |