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Single-Molecule Imaging of Telomerase RNA Reveals a Recruitment-Retention Model for Telomere Elongation.

Laprade H, Querido E, Smith MJ, Guerit D, Crimmins H, Conomos D, Pourret E, Chartrand P, Sfeir A
Mol Cell. 2020 Jul 2;79(1):115-126.e6. doi: 10.1016/j.molcel.2020.05.005. Epub 2020 Jun 3. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
182588pHage2-EF1a-mCherryTRF1-IRES-Blastexpresses mcherry-trf1 in mammalian cells
182589pHage2-EF1a-TagBFP-hCoilinexpresses bfp-hcoilin in mammalian cells
182590pHage2-EF1a-TERT-IRES-Puroexpresses TERT in mammalian cells
182591pHage2-EF1a-TERT K78E-IRES-Puroexpresses TERT K78E in mammalian cells
182592pcDNA3.1(Hygro) TagBFP-hNucleolinexpresses bfp-hNucleolin in mammalian cells
182593pWZL hygro-hTCAB1expresses hTCAB1 in mammalian cells
198862pTopo_Ex2Donor_pPGK-PURODonor template for CRISPR/Cas9 targeting of TERT Ex2
198863pSpCas9_gRNA1TERTKO_BsagRNA5tertko_2A-GFPPlasmid encoding for 2 gRNAs targeting the human TERT gene

Antibodies from Article