Single-Molecule Imaging of Telomerase RNA Reveals a Recruitment-Retention Model for Telomere Elongation.
Laprade H, Querido E, Smith MJ, Guerit D, Crimmins H, Conomos D, Pourret E, Chartrand P, Sfeir A
Mol Cell. 2020 Jul 2;79(1):115-126.e6. doi: 10.1016/j.molcel.2020.05.005. Epub 2020 Jun 3.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 182588 | pHage2-EF1a-mCherryTRF1-IRES-Blast | expresses mcherry-trf1 in mammalian cells |
| 182589 | pHage2-EF1a-TagBFP-hCoilin | expresses bfp-hcoilin in mammalian cells |
| 182590 | pHage2-EF1a-TERT-IRES-Puro | expresses TERT in mammalian cells |
| 182591 | pHage2-EF1a-TERT K78E-IRES-Puro | expresses TERT K78E in mammalian cells |
| 182592 | pcDNA3.1(Hygro) TagBFP-hNucleolin | expresses bfp-hNucleolin in mammalian cells |
| 182593 | pWZL hygro-hTCAB1 | expresses hTCAB1 in mammalian cells |
| 198862 | pTopo_Ex2Donor_pPGK-PURO | Donor template for CRISPR/Cas9 targeting of TERT Ex2 |
| 198863 | pSpCas9_gRNA1TERTKO_BsagRNA5tertko_2A-GFP | Plasmid encoding for 2 gRNAs targeting the human TERT gene |