Two-stage dynamic deregulation of metabolism improves process robustness & scalability in engineered E. coli.
Ye Z, Li S, Hennigan JN, Lebeau J, Moreb EA, Wolf J, Lynch MD
Metab Eng. 2021 Nov;68:106-118. doi: 10.1016/j.ymben.2021.09.009. Epub 2021 Sep 30.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 71326 | pSMART-Ala2 | NADPH dependent Alanine Dehydrogenase, Low Phosphate Dependent E. coli Expression |
| 71348 | pCASCADE-gltA1-gltA2 | gltA1-gltA2 targeting guide RNA E. coli , Low Phosphate Induction |
| 87172 | pSMART-ala11 | NADPH dependent Alanine Dehydrogenase, Constitutive E. coli Expression |
| 87173 | pSMART-ala12 | NADPH dependent Alanine Dehydrogenase, Constitutive E. coli Expression |
| 87174 | pSMART-ala13 | NADPH dependent Alanine Dehydrogenase, Constitutive E. coli Expression |