mtIF3 is locally translated in axons and regulates mitochondrial translation for axonal growth.
Lee S, Park D, Lim C, Kim JI, Min KT
BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1186/s12915-021-01215-w.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 182367 | pAAV2-mtIF3 shRNA_TagRFP657 | shRNA targeting mtIF3 mRNA |
| 182368 | pDendra2-5'UTR(GAPDH)-Dendra2-3'UTR(GAPDH) | Dendra2-based translation reporter |
| 182369 | pcDNA6-5'UTR(mtIF3)-Dendra2-3'UTR(mtIF3) | Dendra2-based translation reporter |
| 182370 | pcDNA6-5'UTR(mtIF3)-mtIF3-Dendra2-3'UTR(mtIF3) | Dendra2-based translation reporter |
| 182371 | pcDNA6-mtIF3-Dendra2 | Dendra2-based translation reporter |
| 182373 | pcDNA6-MRPL50-VC155 | mVenus-based mito-riboBiFC vector (Negative control) |
| 182374 | pcDNA6-MRPS6-VN172 | mVenus-based mito-riboBiFC vector |
| 182375 | pcDNA6-MRPS16-VN172 | mVenus-based mito-riboBiFC vector (Negative control) |
| 182376 | psiCheck2-SV40p-3xFLAG-CMVp-mito-mTFP1 | Dual expression construct encoding 3xFLAG and mito-mTFP1 from separate promoters |
| 182377 | psiCheck2-SV40p-3xFLAG-HSVTKp-mCherry | Dual expression construct encoding 3xFLAG and mCherry from separate promoters |
| 182378 | psiCheck2-SV40p-5'UTR-mtIF3(SR)-3xFLAG-3'UTR-CMVp-mito-mTFP1 | Dual expression construct encoding shRNA-resistant mtIF3 and mito-mTFP1 from separate promoters |
| 182379 | psiCheck2-SV40p-5'UTR-mtIF3(SR)-3xFLAG-3'UTR-HSVTKp-mCherry | Dual expression construct encoding shRNA-resistant mtIF3 and mCherry from separate promoters |
| 182380 | psiCheck2-SV40p-mtIF3(SR)-3xFLAG-CMVp-mito-mTFP1 | Dual expression construct encoding shRNA-resistant mtIF3 and mito-mTFP1 from separate promoters |
| 182381 | psiCheck2-SV40p-mtIF3(SR)-3xFLAG-HSVTKp-mCherry | Dual expression construct encoding shRNA-resistant mtIF3 and mCherry from separate promoters |