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In situ probe and inhibitory RNA synthesis using streamlined gene cloning with Gibson assembly.

Wolff A, Wagner C, Wolf J, Lobo D
STAR Protoc. 2022 Jun 14;3(3):101458. doi: 10.1016/j.xpro.2022.101458. eCollection 2022 Sep 16. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
182263pDL1Gibson cloning vector for synthesis of single and double stranded RNA
182264pAW2-Smed-Slit-1dsRNA and riboprobe synthesis for Schmidtea mediterranea Slit-1

Antibodies from Article