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RFX transcription factors control a miR-150/PDAP1 axis that restrains the proliferation of human T cells.

Chirichella M, Bianchi N, Dzafo E, Foli E, Gualdrini F, Kenyon A, Natoli G, Monticelli S
PLoS Biol. 2022 Feb 10;20(2):e3001538. doi: 10.1371/journal.pbio.3001538. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
182249pmiRGLO Dual-Luciferase-HNRNPAB-3'UTRHuman HNRNPAB 3` UTR region containing 2x binding sites for miR-150 cloned downstream of luciferase reporter gene
182250pmiRGLO Dual-Luciferase-MYB-3'UTRHuman MYB 3` UTR region containing 3x binding sites for miR-150 cloned downstream of luciferase reporter gene
182251pmiRGLO Dual-Luciferase-PDAP1-3'UTRHuman PDAP1 3` UTR region containing 4x binding sites for miR-150 cloned downstream of luciferase reporter gene
182252pmiRGLO Dual-Luciferase-PIK3R1-3'UTRHuman PIK3R1 3` UTR region containing 1x binding site for miR-150 cloned downstream of luciferase reporter gene
182253pmiRGLO Dual-Luciferase-PDAP1-3'UTR-Mut1xHuman PDAP1 3` UTR region mutated in 1x binding site for miR-150 cloned downstream of luciferase reporter gene
182254pmiRGLO Dual-Luciferase-PDAP1-3'UTR-Mut2xHuman PDAP1 3` UTR region mutated in 2x binding site for miR-150 cloned downstream of luciferase reporter gene
182255pmiRGLO Dual-Luciferase-PDAP1-3'UTR-Mut3xHuman PDAP1 3` UTR region mutated in 3x binding site for miR-150 cloned downstream of luciferase reporter gene
182256pmiRGLO Dual-Luciferase-PDAP1-3'UTR-Mut4xHuman PDAP1 3` UTR region mutated in 4x binding site for miR-150 cloned downstream of luciferase reporter gene

Antibodies from Article