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NMDA Receptors in the Lateral Preoptic Hypothalamus Are Essential for Sustaining NREM and REM Sleep.

Miracca G, Anuncibay-Soto B, Tossell K, Yustos R, Vyssotski AL, Franks NP, Wisden W
J Neurosci. 2022 May 31. pii: JNEUROSCI.0350-21.2022. doi: 10.1523/JNEUROSCI.0350-21.2022. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
182246pAAV-iCre-2A-cherry Expresses iCre linked to mCherry. The promoter is a fragment of the hdc gene promoter which drives pan neuronal expression.
182499pAAV-iCre-2A-venusExpresses iCre-venus. The promoter is a fragment of the hdc gene promoter that gives pan neuronal expression
182502pAAV-flex-GFP-shRNA-GluN1Expresses shRNA against mouse NMDA receptor NR1 subunit. Flexed cassette driven by hdc (pan neuronal) gene promoter
182503pAAV-flex-GFP-shRNA-scrambleExpresses scramble shRNA for control expts. Flexed cassette driven by pan neuronal gene promoter
184331pAAV-HDC-DIO-GFP-shRNA.scrambleExpresses scramble shRNA for control expts. Flexed (DIO) cassette driven by hdc promoter fragment for pan neuronal expression

Antibodies from Article