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zMADM (zebrafish mosaic analysis with double markers) for single-cell gene knockout and dual-lineage tracing.

Xu B, Kucenas S, Zong H
Proc Natl Acad Sci U S A. 2022 Mar 1;119(9). pii: 2122529119. doi: 10.1073/pnas.2122529119. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
182153pTol2-eab2-AAExpression of artificial intron separated mApple
182154pTol2-eab2-GGExpression of artificial intron separated eGFP
182155pTol2-eab2-AGExpression of chimeric AG (N-mApple-intron-C-eGFP)
182156pTol2-eab2-GAExpression of chimeric GA (N-eGFP-intron-C-mApple)
182157KI-eab2-AAKnockin plasmid with homologous arm for making zMADM-AA zebrafish line
182158KI-eab2-GGKnockin plasmid with homologous arm for making zMADM-GG zebrafish line
182159KI-eab2-AGKnockin plasmid with homologous arm for making zMADM-AG zebrafish line
182160KI-eab2-GAKnockin plasmid with homologous arm for making zMADM-GA zebrafish line

Antibodies from Article