High-throughput functional variant screens via in vivo production of single-stranded DNA.
Schubert MG, Goodman DB, Wannier TM, Kaur D, Farzadfard F, Lu TK, Shipman SL, Church GM
Proc Natl Acad Sci U S A. 2021 May 4;118(18). pii: 2018181118. doi: 10.1073/pnas.2018181118.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 182131 | pMS_366 | expresses retron RNA, ec.86 Reverse Transcriptase, and CspRecT SSAP, to create a specific rifampicin resistance mutation |
| 182132 | pMS_375 | expresses retron RNA, ec.86 Reverse Transcriptase, CspRecT SSAP, and mutL* to create a specific rifampicin resistance mutation while inhibiting MMR |