Optimization of Nuclear Localization Signal Composition Improves CRISPR-Cas12a Editing Rates in Human Primary Cells.
Luk K, Liu P, Zeng J, Wang Y, Maitland SA, Idrizi F, Ponnienselvan K, Iyer S, Zhu LJ, Luban J, Bauer DE, Wolfe SA
GEN Biotechnol. 2022 Jun;1(3):271-284. doi: 10.1089/genbio.2022.0003. Epub 2022 Jun 14.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 182120 | 2xNLS-NLP-cMyc AspCas12a | pET21a protein expression vector for 2xNLS-NLP-cMyc AspCas12a in bacteria |
| 182121 | 1N/2CxNLS-cMyc-NLP-cMyc AspCas12a | pET21a protein expression vector for 1N/2CxNLS-cMyc-NLP-cMyc AspCas12a in bacteria |
| 182122 | 3xNLS-NLP-cMyc-cMyc AspCas12a | pET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc AspCas12a in bacteria |
| 182123 | 2xNLS-NLP-cMyc LbaCas12a | pET21a protein expression vector for 2xNLS-NLP-cMyc LbaCas12a in bacteria |
| 182124 | 1N/2CxNLS-cMyc-NLP-cMyc LbaCas12a | pET21a protein expression vector for 1N/2CxNLS-cMyc-NLP-cMyc LbaCas12a in bacteria |
| 182125 | 3xNLS-NLP-cMyc-cMyc LbaCas12a | pET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc LbaCas12a in bacteria |
| 182126 | 1N/2CxNLS-cMyc-NLP-cMyc enAspCas12a | pET21a protein expression vector for 1N/2CxNLS-cMyc-NLP-cMyc enAspCas12a in bacteria |
| 182127 | 3xNLS-NLP-cMyc-cMyc enAspCas12a | pET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc enAspCas12a in bacteria |