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O-GlcNAcylation of Sox2 at threonine 258 regulates the self-renewal and early cell fate of embryonic stem cells.

Kim DK, Lee JS, Lee EY, Jang H, Han S, Kim HY, Hwang IY, Choi JW, Shin HM, You HJ, Youn HD, Jang H
Exp Mol Med. 2021 Nov;53(11):1759-1768. doi: 10.1038/s12276-021-00707-7. Epub 2021 Nov 24. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
182046pCAG-FLAG-IRES-blasticidin-Sox2 WTexpression of Sox2
182047pCAG-FLAG-IRES-blasticidin-Sox2 S248Aexpression of Sox2 mutants
182048pCAG-FLAG-IRES-blasticidin-Sox2 T258Aexpression of Sox2 mutants
182049pCAG-FLAG-IRES-blasticidin-Sox2 S248A/T258Aexpression of Sox2 mutants

Antibodies from Article