Preparation of Cas9 Ribonucleoproteins for Genome Editing.
Lin SW, Nguyen VQ, Lin S
Bio Protoc. 2022 May 20;12(10):e4420. doi: 10.21769/BioProtoc.4420. eCollection 2022 May 20.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
182032 | pSL103-SpCas9-FLAG-3XNLS | Expression of recombinant Streptococcus pyogenes Cas9 protein carrying C-terminal FLAG and 3XNLS for genome editing as Cas9 RNP |