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Preparation of Cas9 Ribonucleoproteins for Genome Editing.

Lin SW, Nguyen VQ, Lin S
Bio Protoc. 2022 May 20;12(10):e4420. doi: 10.21769/BioProtoc.4420. eCollection 2022 May 20. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
182032pSL103-SpCas9-FLAG-3XNLSExpression of recombinant Streptococcus pyogenes Cas9 protein carrying C-terminal FLAG and 3XNLS for genome editing as Cas9 RNP

Antibodies from Article