Integrated genome and tissue engineering enables screening of cancer vulnerabilities in physiologically relevant perfusable ex vivo cultures.

Hu M, Lei XY, Larson JD, McAlonis M, Ford K, McDonald D, Mach K, Rusert JM, Wechsler-Reya RJ, Mali P
Biomaterials. 2022 Jan;280:121276. doi: 10.1016/j.biomaterials.2021.121276. Epub 2021 Dec 2. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
182133	Pan-Druggable Cancer Library	All-in-one CRISPR/Cas system with 74 individual elements. 63 elements target cancer-related genes with FDA-approved drug treatments, and 3 elements target key medulloblastoma driver genes.
183267	CROP_C9_Puro_ABL1	All-in-One CRISPRko system with a guide RNA that targets ABL1 gene
183268	CROP_C9_Puro_ABL2	All-in-One CRISPRko system with a guide RNA that targets ABL2 gene
183269	CROP_C9_Puro_ADA	All-in-One CRISPRko system with a guide RNA that targets ADA gene
183270	CROP_C9_Puro_ALK	All-in-One CRISPRko system with a guide RNA that targets ALK gene
183271	CROP_C9_Puro_AR	All-in-One CRISPRko system with a guide RNA that targets AR gene
183272	CROP_C9_Puro_BCL2	All-in-One CRISPRko system with a guide RNA that targets BCL2 gene
183273	CROP_C9_Puro_BRAF	All-in-One CRISPRko system with a guide RNA that targets BRAF gene
183274	CROP_C9_Puro_BTK	All-in-One CRISPRko system with a guide RNA that targets BTK gene
183275	CROP_C9_Puro_CDK4	All-in-One CRISPRko system with a guide RNA that targets CDK4 gene
183276	CROP_C9_Puro_CDK6	All-in-One CRISPRko system with a guide RNA that targets CDK6 gene
183277	CROP_C9_Puro_CSF1R	All-in-One CRISPRko system with a guide RNA that targets CSF1R gene
183278	CROP_C9_Puro_CSF3R	All-in-One CRISPRko system with a guide RNA that targets CSF3R gene
183279	CROP_C9_Puro_CYP17A1	All-in-One CRISPRko system with a guide RNA that targets CYP17A1 gene
183280	CROP_C9_Puro_CYP19A1	All-in-One CRISPRko system with a guide RNA that targets CYP19A1 gene
183281	CROP_C9_Puro_DHFR	All-in-One CRISPRko system with a guide RNA that targets DHFR gene
183282	CROP_C9_Puro_DNMT1	All-in-One CRISPRko system with a guide RNA that targets DNMT1 gene
183283	CROP_C9_Puro_DNMT3A	All-in-One CRISPRko system with a guide RNA that targets DNMT3A gene
183284	CROP_C9_Puro_DNMT3B	All-in-One CRISPRko system with a guide RNA that targets DNMT3B gene
183285	CROP_C9_Puro_EGFR	All-in-One CRISPRko system with a guide RNA that targets EGFR gene
183286	CROP_C9_Puro_EPHA2	All-in-One CRISPRko system with a guide RNA that targets EPHA2 gene
183287	CROP_C9_Puro_ERBB2	All-in-One CRISPRko system with a guide RNA that targets ERBB2 gene
183288	CROP_C9_Puro_ESR1	All-in-One CRISPRko system with a guide RNA that targets ESR1 gene
183289	CROP_C9_Puro_FGFR1	All-in-One CRISPRko system with a guide RNA that targets FGFR1 gene
183290	CROP_C9_Puro_FGFR2	All-in-One CRISPRko system with a guide RNA that targets FGFR2 gene
183291	CROP_C9_Puro_FGFR3	All-in-One CRISPRko system with a guide RNA that targets FGFR3 gene
183292	CROP_C9_Puro_FLT1	All-in-One CRISPRko system with a guide RNA that targets FLT1 gene
183293	CROP_C9_Puro_FLT3	All-in-One CRISPRko system with a guide RNA that targets FLT3 gene
183294	CROP_C9_Puro_FLT4	All-in-One CRISPRko system with a guide RNA that targets FLT4 gene
183295	CROP_C9_Puro_FYN	All-in-One CRISPRko system with a guide RNA that targets FYN gene
183296	CROP_C9_Puro_GART	All-in-One CRISPRko system with a guide RNA that targets GART gene
183297	CROP_C9_Puro_HDAC1	All-in-One CRISPRko system with a guide RNA that targets HDAC1 gene
183298	CROP_C9_Puro_HDAC2	All-in-One CRISPRko system with a guide RNA that targets HDAC2 gene
183299	CROP_C9_Puro_HDAC3	All-in-One CRISPRko system with a guide RNA that targets HDAC3 gene
183300	CROP_C9_Puro_IDH1	All-in-One CRISPRko system with a guide RNA that targets IDH1 gene
183301	CROP_C9_Puro_IDH2	All-in-One CRISPRko system with a guide RNA that targets IDH2 gene
183302	CROP_C9_Puro_JAK1	All-in-One CRISPRko system with a guide RNA that targets JAK1 gene
183303	CROP_C9_Puro_JAK2	All-in-One CRISPRko system with a guide RNA that targets JAK2 gene
183304	CROP_C9_Puro_JAK3	All-in-One CRISPRko system with a guide RNA that targets JAK3 gene
183305	CROP_C9_Puro_KDR	All-in-One CRISPRko system with a guide RNA that targets KDR gene
183306	CROP_C9_Puro_KIT	All-in-One CRISPRko system with a guide RNA that targets KIT gene
183307	CROP_C9_Puro_LCK	All-in-One CRISPRko system with a guide RNA that targets LCK gene
183308	CROP_C9_Puro_MAP2K1	All-in-One CRISPRko system with a guide RNA that targets MAP2K1 gene
183309	CROP_C9_Puro_MAP2K2	All-in-One CRISPRko system with a guide RNA that targets MAP2K2 gene
183310	CROP_C9_Puro_MET	All-in-One CRISPRko system with a guide RNA that targets MET gene
183311	CROP_C9_Puro_MTOR	All-in-One CRISPRko system with a guide RNA that targets MTOR gene
183312	CROP_C9_Puro_PARP1	All-in-One CRISPRko system with a guide RNA that targets PARP1 gene
183313	CROP_C9_Puro_PDGFRA	All-in-One CRISPRko system with a guide RNA that targets PDGFRA gene
183314	CROP_C9_Puro_PDGFRB	All-in-One CRISPRko system with a guide RNA that targets PDGFRB gene
183315	CROP_C9_Puro_PIGF	All-in-One CRISPRko system with a guide RNA that targets PIGF gene
183316	CROP_C9_Puro_PIK3CD	All-in-One CRISPRko system with a guide RNA that targets PIK3CD gene
183317	CROP_C9_Puro_PSMB5	All-in-One CRISPRko system with a guide RNA that targets PSMB5 gene
183318	CROP_C9_Puro_RARA	All-in-One CRISPRko system with a guide RNA that targets RARA gene
183319	CROP_C9_Puro_RET	All-in-One CRISPRko system with a guide RNA that targets RET gene
183320	CROP_C9_Puro_ROS1	All-in-One CRISPRko system with a guide RNA that targets ROS1 gene
183321	CROP_C9_Puro_SMO	All-in-One CRISPRko system with a guide RNA that targets SMO gene
183322	CROP_C9_Puro_SRC	All-in-One CRISPRko system with a guide RNA that targets SRC gene
183323	CROP_C9_Puro_TOP1	All-in-One CRISPRko system with a guide RNA that targets TOP1 gene
183324	CROP_C9_Puro_TOP2A	All-in-One CRISPRko system with a guide RNA that targets TOP2A gene
183325	CROP_C9_Puro_TOP2B	All-in-One CRISPRko system with a guide RNA that targets TOP2B gene
183326	CROP_C9_Puro_TYMS	All-in-One CRISPRko system with a guide RNA that targets TYMS gene
183327	CROP_C9_Puro_VEGFA	All-in-One CRISPRko system with a guide RNA that targets VEGFA gene
183328	CROP_C9_Puro_VEGFB	All-in-One CRISPRko system with a guide RNA that targets VEGFB gene
183329	CROP_C9_Puro_YES1	All-in-One CRISPRko system with a guide RNA that targets YES1 gene
183330	CROP_C9_Puro_MYC	All-in-One CRISPRko system with a guide RNA that targets MYC gene
183337	CROP_C9_Puro_AAVS1A	All-in-One CRISPRko system with a guide RNA that targets the AAVS1 locus
183338	CROP_C9_Puro_AAVS1B	All-in-One CRISPRko system with a guide RNA that targets the AAVS1 locus