Live-Cell Fluorescence Lifetime Multiplexing Using Synthetic Fluorescent Probes.

Frei MS, Koch B, Hiblot J, Johnsson K
ACS Chem Biol. 2022 Jun 17;17(6):1321-1327. doi: 10.1021/acschembio.2c00041. Epub 2022 May 18. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
181998	pcDNA5/FRT/TO_H2B-STf	CMV overexpression of SNAP-tag fast fusion to H2B for nuclear localization in mammalian cell lines
181999	pcDNA5/FRT/TO_STf_LamB1	CMV overexpression of SNAP-tag fast fusion to Lamin B1 for nuclear envelope localization in mammalian cell lines
182000	pcDNA5/FRT/TO_STf	CMV overexpression of SNAP-tag fast in mammalian cell lines
182001	pcDNA5/FRT/TO_NES_STf	CMV overexpression of SNAP-tag fast strictly in the cytoplasm of mammalian cell lines
182002	pcDNA5/FRT/TO_TOMM20-STf	CMV overexpression of SNAP-tag fast at the mitochondria surface of mammalian cell lines
182003	pcDNA5/FRT/TO_[Cox8a]x2_STf	CMV overexpression of SNAP-tag fast in the mitochondrial matrix of mammalian cell lines
182004	pcDNA5/FRT/TO_CalR_STf_KDEL	CMV overexpression of SNAP-tag fast in the endoplasmic reticulum of mammalian cell lines
182005	pcDNA5/FRT/TO_b4g-STf	CMV overexpression of SNAP-tag fast in the golgi apparatus of mammalian cell lines
182006	pcDNA5/FRT/TO_LAMP1_STf	CMV overexpression of SNAP-tag fast in the lysosome of mammalian cell lines
182007	pcDNA5/FRT/TO_STf_SKL	CMV overexpression of SNAP-tag fast in the peroxisome of mammalian cell lines
182008	pcDNA5/FRT/TO_Lyn11-STf	CMV overexpression of SNAP-tag fast at the inner leaflet of the plasma membrane of mammalian cell lines
182009	pcDNA5/FRT/TO_IgKchL_HA_STf_myc_PDGFRtmb	CMV overexpression of SNAP-tag fast at the outer leaflet of the plasma membrane of mammalian cell lines
182010	pcDNA5/FRT/TO_Cep41-STf	CMV overexpression of SNAP-tag fast in mammalian cell lines associated to microtubules
182011	pcDNA5/FRT/TO_Lifeact_STf	CMV overexpression of SNAP-tag fast in mammalian cell lines associated to actin
182012	pCDNA5/FRT/TO_CalR-HaloTag7-P30-SNAP-tag-KDEL	CMV overexpression of HaloTag7 and SNAP-tag fusion in the endoplasmic reticulum of mammalian cell lines