A genome-scale screen for synthetic drivers of T cell proliferation.
Legut M, Gajic Z, Guarino M, Daniloski Z, Rahman JA, Xue X, Lu C, Lu L, Mimitou EP, Hao S, Davoli T, Diefenbach C, Smibert P, Sanjana NE
Nature. 2022 Mar;603(7902):728-735. doi: 10.1038/s41586-022-04494-7. Epub 2022 Mar 16.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 181970 | pOT_1 - lenti-EFS-LTBR-2A-puro | Expresses human LTBR linked to puromycin resistance via P2A for lentiviral delivery. |
| 181971 | pOT_2 - lenti-EFS-tNGFR-2A-puro | Expresses human truncated NGFR linked to puromycin resistance via P2A for lentiviral delivery. |
| 181972 | pOT_3 - lenti-EFS-FMC6.3-28z-2A-puro-2A-LTBR | Expresses anti-CD19 CAR (with CD28 signaling domain) linked to puromycin resistance via P2A and to human LTBR via T2A for lentiviral delivery. |
| 181973 | pOT_4 - lenti-EFS-FMC6.3-BBz-2A-puro-2A-LTBR | Expresses anti-CD19 CAR (with 4-1BB signaling domain) linked to puromycin resistance via P2A and to human LTBR via T2A for lentiviral delivery. |
| 181974 | pOT_5 - lenti-EFS-FMC6.3-28z-2A-puro-2A-tNGFR | Expresses anti-CD19 CAR (with CD28 signaling domain) linked to puromycin resistance via P2A and to human truncated NGFR via T2A for lentiviral delivery. |
| 181975 | pOT_6 - lenti-EFS-FMC6.3-BBz-2A-puro-2A-tNGFR | Expresses anti-CD19 CAR (with 4-1BB signaling domain) linked to puromycin resistance via P2A and to human truncated NGFR via T2A for lentiviral delivery. |