Mouse Heterochromatin Adopts Digital Compaction States without Showing Hallmarks of HP1-Driven Liquid-Liquid Phase Separation.

Erdel F, Rademacher A, Vlijm R, Tunnermann J, Frank L, Weinmann R, Schweigert E, Yserentant K, Hummert J, Bauer C, Schumacher S, Al Alwash A, Normand C, Herten DP, Engelhardt J, Rippe K
Mol Cell. 2020 Apr 16;78(2):236-249.e7. doi: 10.1016/j.molcel.2020.02.005. Epub 2020 Feb 25. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
181883	pPHR-mCherry-mHP1alpha	Soluble light-dependent effector protein (mouse HP1alpha) for optodroplet formation or recruitment to CIBN localizers
181884	pPHR-mCherry-hHP1alpha	Soluble light-dependent effector protein (human HP1alpha) for optodroplet formation or recruitment to CIBN localizers
181885	pPHR-mCherry-hHP1gamma	Soluble light-dependent effector protein (human HP1gamma) for optodroplet formation or recruitment to CIBN localizers
181886	pPHR-mCherry-hNCL	Soluble light-dependent effector protein (nucleolin) for optodroplet formation or recruitment to CIBN localizers
181887	pPHR-mCherry-hNPM	Soluble light-dependent effector protein (nucleophosmin) for optodroplet formation or recruitment to CIBN localizers
181888	pPHR-mCherry-MECP2	Soluble light-dependent effector protein (Methyl-CpG binding protein) for optodroplet formation or recruitment to CIBN localizers
181889	pPHR-mCherry-mHP1alpha-I163A	Soluble light-dependent effector protein (HP1alpha with I163A mutation) for optodroplet formation or recruitment to CIBN localizers,
181890	pPHR-mCherry-nE-mHP1alpha	Soluble light-dependent effector protein (HP1alpha, phosphomimetic NTE mutant) for optodroplet formation or recruitment to CIBN localizers
181891	pPHR-mCherry-nE-mHP1alpha-delCTE	Soluble light-dependent effector protein (HP1alpha, phosphomimetic NTE mutant with deletion of C-terminal extension)for optodroplet formation or recruitment to CIBN localizers
181892	pPHR-mCherry-FUSN-mHP1alpha	Soluble light-dependent effector protein (HP1alpha fused to the N-terminal domain of the fused in sarcoma protein (FUSN) ) for optodroplet formation or recruitment to CIBN localizers
181893	pPHR-mCherry-RGG-GFP-RGG-mHP1alpha	Soluble light-dependent effector protein (HP1alpha fused to the arginine/glycine-rich RGG domain of LAF-1) for optodroplet formation or recruitment to CIBN localizers
181894	pPHR-mCherry-PGL-1-mHP1alpha	Soluble light-dependent effector protein (HP1alpha fused to PGL-1) for optodroplet formation or recruitment to CIBN localizers
181895	pGFP-nE-mHP1alpha	Fluorescently tagged HP1alpha, serines in N-terminal extension (NTE) replaced by glutamates
181896	pGFP-nE-mHP1alpha-delCTE	Fluorescently tagged HP1alpha, serines in N-terminal extension (NTE) replaced by glutamates, C-terminal deletion
181897	pGFP-FUSN-mHP1alpha	Fluorescently tagged HP1alpha fused to the N-terminal domain of the fused in sarcoma protein (FUSN)
181898	pRGG-GFP-RGG-mHP1alpha	Fluorescently tagged HP1alpha fused to the arginine/glycine-rich RGG domain of LAF-1
181899	pGFP-PGL-1-mHP1alpha	Fluorescently tagged HP1alpha fused to PGL-1
181900	pGFP-mHP1alpha	Fluorescently tagged HP1alpha
181901	pGFP-mHP1beta	Fluorescently tagged HP1beta
181902	pGFP-mHP1gamma	Fluorescently tagged HP1gamma
181903	pGFP-hPMLIII	Fluorescently tagged PMLIII
181904	pMECP2-GFP	Fluorescently tagged MECP2, GFP
181905	pMECP2-RFP	Fluorescently tagged MECP2, RFP
181906	pdCas9-GFP	Fluorescently tagged dead Cas9
181907	pdCas9-GFP-VPR	Fluorescently tagged dead Cas9 fused to the tripartite transcriptional activator VP64-p65-Rta
181908	psgRNA-2xMS2-mSAT	Single guide RNA with 2XMS2 loops targeting mouse major satellite repeats
181909	psgRNA-2xMS2-lacO	Single guide RNA with 2XMS2 loops targeting bacterial lac operator
181910	pET16-mHP1alpha	Bacterial protein expression vector for HP1alpha
181911	pET28-mHP1alpha	Bacterial protein expression vector for HP1alpha
181912	pET28-GFP-mHP1alpha	Bacterial protein expression vector for GFP-tagged HP1alpha
181913	pTRE3G-ZSGreen1-mHP1alpha	Inducible mammalian expression vector for ZsGreen1-tagged HP1alpha