DrFLINC Contextualizes Super-resolution Activity Imaging.
Lin W, Mo GCH, Mehta S, Zhang J
J Am Chem Soc. 2021 Sep 22;143(37):14951-14955. doi: 10.1021/jacs.1c05530. Epub 2021 Sep 13.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 182321 | pcDNA3-Lyn-μDpTT | Plasma membrane-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging. |
| 182322 | pcDNA3-Lifeact-μDpTT | Actin-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging. |
| 182323 | pDronpa-N1-Tau-μDpTT | Microtubule-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging. |
| 182324 | pcDNA3-DAKAP-μDpTT | Mitochondria-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging. |
| 182325 | pcDNA3-CYP450-μDpTT | Endoplasmic reticulum-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging. |
| 182326 | pcDNA3-β2AR-μDpTT | Beta-2 adrenergic receptor-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging. |
| 182327 | pcDNA3-RIIα-μDpTT | PKA RIIα subunit-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging. |
| 182328 | pcDNA3-H2B-μDpTT | Histone H2B-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging. |
| 182329 | pcDNA3-Dronpa-Kras | Plasma membrane-targeted Dronpa. Fluctuating green-fluorescent label for live-cell super-resolution imaging. |
| 182330 | pcDNA3-Lifeact-Dronpa | Actin-targeted Dronpa. Fluctuating green-fluorescent label for live-cell super-resolution imaging. |
| 182331 | pcDNA3-RIIα-Dronpa | PKA RIIα subunit-targeted Dronpa. Fluctuating green-fluorescent label for live-cell super-resolution imaging. |
| 182332 | pcDNA3-AKAP79-Dronpa | AKAP79-targeted Dronpa. Fluctuating green-fluorescent label for live-cell super-resolution imaging. |
| 182333 | pGW-Dronpa-Cav1.2 | L-type voltage-gated calcium channel α1C subunit tagged with Dronpa. Fluctuating green-fluorescent label for live-cell super-resolution imaging. |
| 182334 | pcDNA3-DrFLINC-AKAR-Kras | Genetically encoded fluorescent biosensor for super-resolution imaging of PKA activity; targeted to the plasma membrane. Compatible with multiplexed super-resolution imaging using a Dronpa-tagged POI. |