A Highly Sensitive Fluorescent Akt Biosensor Reveals Lysosome-Selective Regulation of Lipid Second Messengers and Kinase Activity.
Chen M, Sun T, Zhong Y, Zhou X, Zhang J
ACS Cent Sci. 2021 Dec 22;7(12):2009-2020. doi: 10.1021/acscentsci.1c00919. Epub 2021 Dec 3.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 184047 | pcDNA3-ExRai-AktAR2 | Improved excitation-ratiometric biosensor for monitoring Akt/Protein Kinase B activity in living cells. |
| 184048 | pcDNA3-Cyto-ExRai-AktAR2 | Cytosol-targeted ExRai-AktAR2. |
| 184049 | pcDNA3-Nuc-ExRai-AktAR2 | Nuclear-targeted ExRai-AktAR2. |
| 184050 | pcDNA3-PM-ExRai-AktAR2 | Plasma membrane-targeted ExRai-AktAR2 |
| 184051 | pcDNA3-Golgi-ExRai-AktAR2 | Golgi-targeted ExRai-AktAR2. |
| 184052 | pcDNA3-Lyso-ExRai-AktAR2 | Lysosome-targeted ExRai-AktAR2. |
| 184053 | pcDNA3-Lyso-InPAkt | Lysosome-targeted FRET-based biosensor for monitoring 3-phosphoinositide dynamics in living cells. |
| 184054 | pcDNA3-Lyso-PTEN(A4)-mCherry | Lysosome-targeted constitutively active mutant of phosphatase and tensin homolog (PTEN); tagged with mCherry. |
| 184055 | pcDNA3-Lyso-INPP4B-mCherry | Lysosome targeted inositol polyphosphate-4-phosphatase type II B; tagged with mCherry. |