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Role of Ring6 in the Function of the E. coli MCE Protein LetB.

Vieni C, Coudray N, Isom GL, Bhabha G, Ekiert DC
J Mol Biol. 2022 Jan 22;434(7):167463. doi: 10.1016/j.jmb.2022.167463. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
170393pBEL2116Plasmid for genetic complementation of LetAB with ΔRing 1 LetB mutant
170394pBEL2117Plasmid for genetic complementation of LetAB with ΔRing 2 LetB mutant
170395pBEL2118Plasmid for genetic complementation of LetAB with ΔRing 3 LetB mutant
170396pBEL2119Plasmid for genetic complementation of LetAB with ΔRing 4 LetB mutant
170397pBEL2120Plasmid for genetic complementation of LetAB with ΔRing 5 LetB mutant
170398pBEL2121Plasmid for genetic complementation of LetAB with ΔRing 6 LetB mutant
170399pBEL2122Plasmid for genetic complementation of LetAB with ΔRing 7 LetB mutant
170400pBEL2196For expresson of LetA-[LetB PLL6->1] operon from E. coli. N-terminal his tag on LetA, araBAD promoter
170401pBEL2197For expresson of LetA-[LetB PLL6->3] operon from E. coli. N-terminal his tag on LetA, araBAD promoter
170402pBEL2198For expresson of LetA-[LetB PLL6->5] operon from E. coli. N-terminal his tag on LetA, araBAD promoter
170403pBEL2251For expresson of LetA-[LetB PLL6->2] operon from E. coli. N-terminal his tag on LetA, araBAD promoter
170404pBEL2252For expresson of LetA-[LetB PLL6->4] operon from E. coli. N-terminal his tag on LetA, araBAD promoter
170405pBEL2253For expresson of LetA-[LetB PLL6->7] operon from E. coli. N-terminal his tag on LetA, araBAD promoter
170406pBEL2199Genetic complementation plasmid of LetA-[LetB PLL6->1] mutant
170407pBEL2200Genetic complementation plasmid of LetA-[LetB PLL6->3] mutant
170408pBEL2201Genetic complementation plasmid of LetA-[LetB PLL6->5] mutant
170409pBEL2254Genetic complementation plasmid of LetA-[LetB PLL6->2] mutant
170410pBEL2255Genetic complementation plasmid of LetA-[LetB PLL6->4] mutant
170411pBEL2256Genetic complementation plasmid of LetA-[LetB PLL6->7] mutant
170412pBEL1863Genetic complementation plasmid of LetAB with LetB mutant A473N
170413pBEL1864Genetic complementation plasmid of LetAB with LetB mutant W476N
170414pBEL1865Genetic complementation plasmid of LetAB with LetB mutant I477N
170415pBEL1866Genetic complementation plasmid of LetAB with LetB mutant L595N
170416pBEL1867Genetic complementation plasmid of LetAB with LetB mutant A598N
170417pBEL1868Genetic complementation plasmid of LetAB with LetB mutant L599N
170418pBEL1869Genetic complementation plasmid of LetAB with LetB mutant L711N
170419pBEL1870Genetic complementation plasmid of LetAB with LetB mutant I714N
170420pBEL1871Genetic complementation plasmid of LetAB with LetB mutant L715N
170421pBEL1872Genetic complementation plasmid of LetAB with LetB mutant F830N
170422pBEL1873Genetic complementation plasmid of LetAB with LetB mutant F833N
170423pBEL1874Genetic complementation plasmid of LetAB with LetB mutant I834N
170424pBEL2343Genetic complementation plasmid of LetAB with LetB mutant L123N
170425pBEL2344Genetic complementation plasmid of LetAB with LetB mutant L125N
170426pBEL2345Genetic complementation plasmid of LetAB with LetB mutant V126N
175960pBEL1284For expression of LetA-LetB operon from E. coli. N-terminal his tag on LetA, araBAD promoter
175961pBEL2114For expression of LetA-[ΔRing6 LetB] operon from E. coli. N-terminal his tag on LetA, araBAD promoter
175962pBEL2265For expresson of LetA-[LetB PLL3->6] operon from E. coli. N-terminal his tag on LetA, araBAD promoter
175963pBEL2268Genetic complementation plasmid of LetA-[LetB PLL3->6] mutant
175964pBEL2409Genetic complementation plasmid of LetAB with 6PLL3-helix
175965pBEL2412Genetic complementation plasmid of LetAB with 6PLL3-hairpin
175966pBEL2414Genetic complementation plasmid of LetAB with LetB(P701N)
175967pBEL2415Genetic complementation plasmid of LetAB with LetB(I703N)
175968pBEL2416Genetic complementation plasmid of LetAB with LetB(A705N)
175969pBEL2417Genetic complementation plasmid of LetAB with LetB(A706N)
175970pBEL2418Genetic complementation plasmid of LetAB with LetB(G707N)
175971pBEL2419Genetic complementation plasmid of LetAB with LetB(V708N)
175972pBEL2420Genetic complementation plasmid of LetAB with LetB(P717N)
181815pBEL1748Expression plasmid for LetB ΔRing 1 (without TM region)
181816pBEL1683Expression plasmid for LetB ΔRing 2 (without TM region)
181817pBEL2307Expression plasmid for LetB ΔRing 3 (without TM region)
181818pBEL2308Expression plasmid for LetB ΔRing 4 (without TM region)
181819pBEL2309Expression plasmid for LetB ΔRing 5 (without TM region)
181820pBEL1749Expression plasmid for LetB ΔRing 7 (without TM region)

Antibodies from Article