A split prime editor with untethered reverse transcriptase and circular RNA template.
Liu B, Dong X, Cheng H, Zheng C, Chen Z, Rodriguez TC, Liang SQ, Xue W, Sontheimer EJ
Nat Biotechnol. 2022 Apr 4. pii: 10.1038/s41587-022-01255-9. doi: 10.1038/s41587-022-01255-9.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 181799 | MCP-M-MLV-RT | MCP-M-MLV-RT (MS2-N55K-MMLVRT) |
| 181801 | MMLV-RT | MMLV-RT (3XFLAG-SV40-MMLVRT) |
| 181802 | U6-petRNA | petRNA cloning plasmid |
| 181803 | petRNA-FANCF-v3' | petRNA FANCF v3' |
| 181804 | pET28a-NLS-M-MLV-RT | pET28a-NLS-M-MLV RT |
| 181805 | maturase-RT | E.r. maturase RT |
| 181806 | GsI-IIC-RT | GsI-IIC RT |
| 181807 | PE-maturase-RT | PE-E.r. maturase RT |
| 181808 | PE-GsI-IIC-RT | PE-GsI-IIC RT |
| 181809 | scFv-M-MLV-RT | scFv-M-MLV RT |
| 181811 | MS2-pegRNA | ms2 incorporated pegRNA-1.1 |