Phase Separation of a PKA Regulatory Subunit Controls cAMP Compartmentation and Oncogenic Signaling.
Zhang JZ, Lu TW, Stolerman LM, Tenner B, Yang JR, Zhang JF, Falcke M, Rangamani P, Taylor SS, Mehta S, Zhang J
Cell. 2020 Sep 17;182(6):1531-1544.e15. doi: 10.1016/j.cell.2020.07.043. Epub 2020 Aug 25.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 181840 | pcDNA3.1(+)-RIα-EGFP | GFP-tagged PKA RIα subunit for mammalian expression. |
| 181841 | pcDNA3.1(+)-PKAcat-mCherry | mCherry-tagged PKA catalytic subunit for mammalian expression. |
| 181842 | pcDNA3.1(+)-RIα(D/D+linker)-EGFP | EGFP-tagged docking/dimerization (D/D) and linker region of PKA RIα; for mammalian expression. |
| 181843 | pcDNA3.1-RIαΔ(D/D+linker)-EGFP | GFP-tagged PKA RIα subunit lacking the docking/dimerization (D/D) and linker regions; for mammalian expression. |
| 181844 | pcDNA3.1(+)-FluoSTEP-AKAR | Genetically encoded FRET-based sensor for monitoring PKA activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence. |
| 181845 | pcDNA3.1(+)-FluoSTEP-ICUE | Genetically encoded FRET-based sensor for monitoring cAMP dynamics near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence. |
| 181846 | pcDNA3-ICUE4 | Fourth-generation FRET-based Indicator of cAMP Using Epac. Genetically encoded cAMP indicator. Contains high-sensitivity Epac Q270E mutation. |
| 181847 | pcDNA3-PDE4D2cat-ICUE4 | ICUE4 cAMP sensor tethered to the catalytic domain of PDE4D2. |
| 181848 | pcDNA3-RIα-ICUE4 | ICUE4 cAMP sensor tethered to the PKA RIα subunit. |
| 181849 | pcDNA3.1(+)-R/G-ICUE | ICUE cAMP sensor using sfGFP and mRuby2 as the FRET donor and acceptor. |
| 181850 | pcDNA3.1(+)-DnaJB1-PKAcat-mCherry | mCherry-tagged DnaJB1-PKA catalytic subunit fusion; for mammalian expression. |
| 181851 | pcDNA3.1(+)-DnaJB1-PKAcat(K72H)-mCherry | mCherry-tagged DnaJB1-PKA catalytic subunit fusion; contains catalytically dead PKA; for mammalian expression. |
| 181852 | pcDNA3.1(+)-PKAcat(G1A)-mCherry | mCherry-tagged PKA catalytic subunit lacking N-terminal myristoylation site; for mammalian expression. |
| 181853 | pcDNA3.1(+)-Myr-DnaJB1-PKAcat-mCherry | mCherry-tagged DnaJB1-PKA catalytic subunit fusion with N-terminal myristoylation site added; for mammalian expression. |
| 181854 | pcDNA3.1(+)-DnaJB1(H33Q)-PKAcat-mCherry | mCherry-tagged DnaJB1-PKA catalytic subunit fusion with H33Q DnaJB1 mutation to block Hsp70 binding; for mammalian expression. |
| 181855 | pcDNA3.1(+)-Myr-DnaJB1(H33Q)-PKAcat-mCherry | mCherry-tagged DnaJB1-PKA catalytic subunit fusion N-terminal myristoylation site and H33Q DnaJB1 mutation to block Hsp70 binding; for mammalian expression. |