Fluorescent Biosensors for Multiplexed Imaging of Phosphoinositide Dynamics.
Hertel F, Li S, Chen M, Pott L, Mehta S, Zhang J
ACS Chem Biol. 2020 Jan 17;15(1):33-38. doi: 10.1021/acschembio.9b00691. Epub 2020 Jan 6.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 181830 | pcDNA3-Lyn-PlcR | Genetically encoded FRET-based sensor for monitoring plasma membrane PI(4,5)P2 dynamics in living cells. |
| 181831 | pcDNA3-Lyn-PlcR(K30/32L) | Phospholipid binding-deficient Lyn-PlcR negative-control plasmid. |
| 181832 | pcDNA3-Lyn-dPlcR | Genetically encoded single-color sensor for monitoring plasma membrane PI(4,5)P2 dynamics in living cells. Based on dimerization-dependent green fluorescent protein (ddGFP). |
| 181833 | pcDNA3-Lyn-dPlcR(K30/32L) | Phospholipid binding-deficient Lyn-dPlcR negative-control plasmid. |
| 181834 | pcDNA3-Lyn-dInPAkt | Genetically encoded single-color sensor for monitoring plasma membrane PI(3,4,5)P2 dynamics in living cells. Based on dimerization-dependent red fluorescent protein (ddRFP). |
| 181835 | pcDNA3-Lyn-dInPAkt(R23/25A) | Phospholipid binding-deficient Lyn-dInPAkt negative-control plasmid. |