Characterizing and Improving pET Vectors for Cell-free Expression.
Jew K, Smith PEJ, So B, Kasman J, Oza JP, Black MW
Front Bioeng Biotechnol. 2022 Jun 23;10:895069. doi: 10.3389/fbioe.2022.895069. eCollection 2022.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 180754 | pET30-T7-lacO-His-sfGFP | pET30 vector with sfGFP-S-tag inserted between the Bgl II and T7 terminator |
| 180755 | pET30-T7-His-sfGFP | pET30 vector missing lacO with sfGFP-S-tag inserted between the Bgl II and T7 terminator |
| 180756 | pET30-T7-lacO-sfGFP | pET30 vector missing N-terminal His tag with sfGFP-S-tag inserted between the Bgl II and T7 terminator |
| 180757 | pET30-T7-sfGFP | pET30 vector missing lacO and N-terminal His tag with sfGFP-S-tag inserted between the Bgl II and T7 terminator |