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A Multiplex MoClo Toolkit for Extensive and Flexible Engineering of Saccharomyces cerevisiae.

Shaw WM, Khalil AS, Ellis T
ACS Synth Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
180654pMYT001Encodes pZ3 as a Type 2 part to be used in the Dueber YTK and Ellis MYT systems
180655pMYT002Encodes pLacD as a Type 2 part to be used in the Dueber YTK and Ellis MYT systems
180656pMYT003Encodes pLacE as a Type 2 part to be used in the Dueber YTK and Ellis MYT systems
180657pMYT004Encodes pLacC as a Type 2 part to be used in the Dueber YTK and Ellis MYT systems
180658pMYT005Encodes pLacFEC as a Type 2 part to be used in the Dueber YTK and Ellis MYT systems
180660pMYT007Encodes pLacFBA1 as a Type 2 part to be used in the Dueber YTK and Ellis MYT systems
180661pMYT008Encodes pLacENO2 as a Type 2 part to be used in the Dueber YTK and Ellis MYT systems
180662pMYT009Encodes pLacSpTDH3 as a Type 2 part to be used in the Dueber YTK and Ellis MYT systems
180663pMYT010Encodes pTet2 as a Type 2 part to be used in the Dueber YTK and Ellis MYT systems
180664pMYT011Encodes pTet3 as a Type 2 part to be used in the Dueber YTK and Ellis MYT systems
180665pMYT012Encodes pTet4 as a Type 2 part to be used in the Dueber YTK and Ellis MYT systems
180666pMYT013Encodes pTet5 as a Type 2 part to be used in the Dueber YTK and Ellis MYT systems
180667pMYT014Encodes pTet6 as a Type 2 part to be used in the Dueber YTK and Ellis MYTK systems
180668pMYT015Encodes pTet7 as a Type 2 part to be used in the Dueber YTK and Ellis MYT systems
180669pMYT016Encodes Z3EV as a Type 3 part to be used in the Dueber YTK and Ellis MYT systems
180670pMYT017Encodes LacI as a Type 3 part to be used in the Dueber YTK and Ellis MYT systems
180671pMYT018Encodes rtTA as a Type 3 part to be used in the Dueber YTK and Ellis MYT systems
180672pMYT019Encodes mScarlet-I as a Type 3 part to be used in the Dueber YTK and Ellis MYT systems
180673pMYT020Encodes mNeonGreen as a Type 3 part to be used in the Dueber YTK and Ellis MYT systems
180674pMYT021Encodes mTagBFP2 as a Type 3 part to be used in the Dueber YTK and Ellis MYT systems
180675pMYT022Encodes mScarlet-I as a Type 4a part to be used in the Dueber YTK and Ellis MYT systems
180676pMYT023Encodes mNeonGreen as a Type 4a part to be used in the Dueber YTK and Ellis MYT systems
180677pMYT024Encodes mTagBFP2 as a Type 4a part to be used in the Dueber YTK and Ellis MYT systems
180678pMYT025Encodes tTDH3 as a Type 4 part to be used in the Dueber YTK and Ellis MYT systems
180679pMYT026Encodes tTEF1 as a Type 4 part to be used in the Dueber YTK and Ellis MYT systems
180680pMYT027Encodes tTDH2 as a Type 4 part to be used in the Dueber YTK and Ellis MYT systems
180681pMYT028Encodes tPDC1 as a Type 4 part to be used in the Dueber YTK and Ellis MYT systems
180682pMYT029Encodes URA3 as a yeast Vector selectable marker to be used in the Ellis MYT system
180683pMYT030Encodes LEU2 as a yeast Vector selectable marker to be used in the Ellis MYT system
180684pMYT031Encodes HIS3 as a yeast Vector selectable marker to be used in the Ellis MYT system
180685pMYT032Encodes TRP1 as a yeast Vector selectable marker to be used in the Ellis MYT system
180686pMYT033Encodes MET17 as a yeast Vector selectable marker to be used in the Ellis MYT system
180687pMYT034Encodes LYS2 as a yeast Vector selectable marker to be used in the Ellis MYT system
180688pMYT035Encodes KanR as a yeast Vector selectable marker to be used in the Ellis MYT system
180689pMYT036Encodes NatR as a yeast Vector selectable marker to be used in the Ellis MYT system
180690pMYT037Encodes HygR as a yeast Vector selectable marker to be used in the Ellis MYT system
180691pMYT038Encodes ZeoR as a yeast Vector selectable marker to be used in the Ellis MYT system
180692pMYT039Assembly cassette with ConLS and ConR1 connectors to be used in the Dueber YTK and Ellis MYT systems
180693pMYT040Assembly cassette with ConL1 and ConRE connectors to be used in the Dueber YTK and Ellis MYT systems
180694pMYT041Assembly cassette with ConL1 and ConR2 connectors to be used in the Dueber YTK and Ellis MYT systems
180695pMYT042Assembly cassette with ConL2 and ConRE connectors to be used in the Dueber YTK and Ellis MYT systems
180696pMYT043Assembly cassette with ConL2 and ConR3 connectors to be used in the Dueber YTK and Ellis MYT systems
180697pMYT044Assembly cassette with ConL3 and ConRE connectors to be used in the Dueber YTK and Ellis MYT systems
180698pMYT045Assembly cassette with ConL3 and ConR4 connectors to be used in the Dueber YTK and Ellis MYT systems
180699pMYT046Assembly cassette with ConL4 and ConRE connectors to be used in the Dueber YTK and Ellis MYT systems
180700pMYT047Assembly cassette with ConL4 and ConR5 connectors to be used in the Dueber YTK and Ellis MYT systems
180701pMYT048Assembly cassette with ConL5 and ConRE connectors to be used in the Dueber YTK and Ellis MYT systems
180702pMYT049Assembly cassette with ConL5 and ConR6 connectors to be used in the Dueber YTK and Ellis MYT systems
180703pMYT050Assembly cassette with ConL6 and ConRE connectors to be used in the Dueber YTK and Ellis MYT systems
180704pMYT051Assembly cassette with ConL6 and ConR7 connectors to be used in the Dueber YTK and Ellis MYT systems
180705pMYT052Assembly cassette with ConL7 and ConRE connectors to be used in the Dueber YTK and Ellis MYT systems
180706pMYT053Assembly cassette with ConL7 and ConR8 connectors to be used in the Dueber YTK and Ellis MYT systems
180707pMYT054Assembly cassette with ConL8 and ConRE connectors to be used in the Dueber YTK and Ellis MYT systems
180708pMYT055Assembly cassette with ConL8 and ConR9 connectors to be used in the Dueber YTK and Ellis MYT systems
180709pMYT056Assembly cassette with ConL9 and ConRE connectors to be used in the Dueber YTK and Ellis MYT systems
180710pMYT057Spacer cassette with ConLS and ConR1 connectors to be used in the Dueber YTK and Ellis MYT systems
180711pMYT058Spacer cassette with ConL1 and ConRE connectors to be used in the Dueber YTK and Ellis MYT systems
180712pMYT059Spacer cassette with ConL1 and ConR2 connectors to be used in the Dueber YTK and Ellis MYT systems
180713pMYT060Spacer cassette with ConL2 and ConRE connectors to be used in the Dueber YTK and Ellis MYT systems
180714pMYT061Spacer cassette with ConL2 and ConR3 connectors to be used in the Dueber YTK and Ellis MYT systems
180715pMYT062Spacer cassette with ConL3 and ConRE connectors to be used in the Dueber YTK and Ellis MYT systems
180716pMYT063Spacer cassette with ConL3 and ConR4 connectors to be used in the Dueber YTK and Ellis MYT systems
180717pMYT064Spacer cassette with ConL4 and ConRE connectors to be used in the Dueber YTK and Ellis MYT systems
180718pMYT065Spacer cassette with ConL4 and ConR5 connectors to be used in the Dueber YTK and Ellis MYT systems
180719pMYT066Spacer cassette with ConL5 and ConRE connectors to be used in the Dueber YTK and Ellis MYT systems
180720pMYT067Spacer cassette with ConL5 and ConR6 connectors to be used in the Dueber YTK and Ellis MYT systems
180721pMYT068Spacer cassette with ConL6 and ConRE connectors to be used in the Dueber YTK and Ellis MYT systems
180722pMYT069Spacer cassette with ConL6 and ConR7 connectors to be used in the Dueber YTK and Ellis MYT systems
180723pMYT070Spacer cassette with ConL7 and ConRE connectors to be used in the Dueber YTK and Ellis MYT systems
180724pMYT071Spacer cassette with ConL7 and ConR8 connectors to be used in the Dueber YTK and Ellis MYT systems
180725pMYT072Spacer cassette with ConL8 and ConRE connectors to be used in the Dueber YTK and Ellis MYT systems
180726pMYT073Spacer cassette with ConL8 and ConR9 connectors to be used in the Dueber YTK and Ellis MYT systems
180727pMYT074Spacer cassette with ConL9 and ConRE connectors to be used in the Dueber YTK and Ellis MYT systems
180728pMYT075Markerless yeast integration Vector at Int.1 locus to used in the Dueber YTK and Ellis MYT systems
180729pMYT076Markerless yeast integration Vector at Int.2 locus to used in the Dueber YTK and Ellis MYT systems
180730pMYT077Markerless yeast integration Vector at Int.3 locus to used in the Dueber YTK and Ellis MYT systems
180731pMYT078Markerless yeast integration Vector at Int.4 locus to used in the Dueber YTK and Ellis MYT systems
180732pMYT079Markerless yeast integration Vector at Int.5 locus to used in the Dueber YTK and Ellis MYT systems
180733pMYT080Markerless yeast integration Vector at Int.6 locus to used in the Dueber YTK and Ellis MYT systems
180734pMYT081Markerless yeast integration Vector at Int.7 locus to used in the Dueber YTK and Ellis MYT systems
180736pMYT083Markerless yeast integration Vector at Int.9 locus to used in the Dueber YTK and Ellis MYT systems
180737pMYT084Markerless yeast integration Vector at Int.10 locus to used in the Dueber YTK and Ellis MYT systems
180738pMYT085Markerless yeast integration Spacer at Int.1 locus to used in the Ellis MYT system
180739pMYT086Markerless yeast integration Spacer at Int.2 locus to used in the Ellis MYT system
180740pMYT087Markerless yeast integration Spacer at Int.3 locus to used in the Ellis MYT system
180741pMYT088Markerless yeast integration Spacer at Int.4 locus to used in the Ellis MYT system
180742pMYT089Markerless yeast integration Spacer at Int.5 locus to used in the Ellis MYT system
180743pMYT090Markerless yeast integration Spacer at Int.6 locus to used in the Ellis MYT system
180744pMYT091Markerless yeast integration Spacer at Int.7 locus to used in the Ellis MYT system
180746pMYT093Markerless yeast integration Spacer at Int.9 locus to used in the Ellis MYT system
180747pMYT094Markerless yeast integration Spacer at Int.10 locus to used in the Ellis MYT system
198864pMYT082Markerless yeast integration Vector at Int.8 locus to be used in the Dueber YTK and Ellis MYT systems
198865pMYT092Markerless yeast integration Spacer at Int.8 locus to be used in the Ellis MYT system
198866pMYT095CRISPR/Cas9 Vector with URA3 selectable marker to be used in the Ellis MYT system
198867pMYT096Transient CRISPR/Cas9 Vector without a selectable marker to be used in the Ellis MYT system
203322pMYT006Encodes pLacHTA1 as a Type 2 part to be used in the Dueber YTK and Ellis MYT systems

Antibodies from Article