Actin chromobody imaging reveals sub-organellar actin dynamics.
Schiavon CR, Zhang T, Zhao B, Moore AS, Wales P, Andrade LR, Wu M, Sung TC, Dayn Y, Feng JW, Quintero OA, Shadel GS, Grosse R, Manor U
Nat Methods. 2020 Sep;17(9):917-921. doi: 10.1038/s41592-020-0926-5. Epub 2020 Aug 10.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 182576 | LifeAct-GFP-Fis1 | Expresses LifeAct-GFP targeted to the outer mitochondrial membrane via the Fis1 tail anchor sequence |
| 182577 | LifeAct-GFP-cytoB5RR | Expresses LifeAct-GFP targeted to the ER via the CytB5RR tail anchor sequence |
| 182579 | mCherry-Cytb5 | Expresses mCherry targeted to the ER via the CytB5RR tail anchor sequence |
| 182580 | mCherry-Fis1 | Expresses mCherry targeted to the outer mitochondrial membrane via the Fis1 tail anchor sequence |