Chimeric CRISPR-CasX enzymes and guide RNAs for improved genome editing activity.
Tsuchida CA, Zhang S, Doost MS, Zhao Y, Wang J, O'Brien E, Fang H, Li CP, Li D, Hai ZY, Chuck J, Brotzmann J, Vartoumian A, Burstein D, Chen XW, Nogales E, Doudna JA, Liu JG
Mol Cell. 2022 Mar 17;82(6):1199-1209.e6. doi: 10.1016/j.molcel.2022.02.002. Epub 2022 Feb 25.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 180509 | pCAT079 | Plasmid expressing mammalian codon optimized wt DpbCasX, sgRNAv2 scaffold, and restriction sites to clone in new spacers |
| 180510 | pCAT105 | Plasmid expressing mammalian codon optimized engineered DpbCasX-R3, sgRNAv2 scaffold, and restriction sites to clone in new spacers |
| 180511 | pCAT077 | Plasmid expressing mammalian codon optimized wt PlmCasX, sgRNAv2 scaffold, and restriction sites to clone in new spacers |
| 180512 | pCAT100 | Plasmid expressing mammalian codon optimized engineered chimeric PlmCasX-R1, sgRNAv2 scaffold, and restriction sites to clone in new spacers |
| 180513 | pCAT526 | Plasmid expressing mammalian codon optimized wt PlmCasX, mNeonGreen, sgRNAv1 scaffold, and restriction sites to clone in new spacers |
| 180514 | pCAT527 | Plasmid expressing mammalian codon optimized engineered chimeric PlmCasX-R1, mNeonGreen, sgRNAv2 scaffold, and restriction sites to clone in new spacers |
| 180605 | pJJGL001 | Plasmid expressing E. coli codon optimized His-MBP-TEV-DpbCasX |
| 180606 | pJJGL002 | Plasmid expressing E. coli codon optimized His-MBP-TEV-PlmCasX |
| 180607 | pJJGL003 | Plasmid expressing E. coli codon optimized His-MBP-TEV-DpbCasX+Region3 insertion |
| 180608 | pJJGL004 | Plasmid expressing E. coli codon optimized His-MBP-TEV-PlmCasX+Region1 insertion |