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Verification of extracellular vesicle-mediated functional mRNA delivery by RNA editing

Somiya M, Kuroda S
bioRxiv 2022.01.25.477620 (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
180501pcDNA3.1-NlucMammalian expression of Nluc
180502pcDNA3.1-Nluc(W12stop)Mammalian expression of Nluc(W12stop)
180503pCMV-CD63-L7AeHuman CD63-L7Ae fusion protein
180504pCIneo-Nluc-2xC/D boxMammalian expression of Nluc-2xC/D box
180505pCIneo-Nluc(W12stop)-2xC/D boxMammalian expression of Nluc(W12stop)-2xC/D box
180506PasCas13b-gRNA-Nluc(W12stop)PasCas13b-gRNA targeting Nluc(W12stop) for RNA editing
180507PasCas13b-gRNA-EGFP(W58stop)PasCas13b-gRNA targeting EGFP(W58stop) for RNA editing
180508pcDNA3.1-EGFP(W58stop)-HiBiTMammalian expression of EGFP(W58stop)-HiBiT

Antibodies from Article