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Dual targeting of brain region-specific kinases potentiates neurological rescue in Spinocerebellar ataxia type 1.

Lee WS, Lavery L, Rousseaux MWC, Rutledge EB, Jang Y, Wan YW, Wu SR, Kim W, Al-Ramahi I, Rath S, Adamski CJ, Bondar VV, Tewari A, Soleimani S, Mota S, Yalamanchili HK, Orr HT, Liu Z, Botas J, Zoghbi HY
EMBO J. 2021 Apr 1;40(7):e106106. doi: 10.15252/embj.2020106106. Epub 2021 Mar 11. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
180379W118-1_eGFPlentiviral transduction of eGFP gene
180380W118-1_hZBTB7Blentiviral transduction of human ZBTB7B gene
180381W118-1_hZBTB7B_R363Llentiviral transduction of human ZBTB7B gene with a point mutation (R363L) in the first ZF domain
180382W118-1_hZBTB7B_R49Hlentiviral transduction of human ZBTB7B gene with a point mutation (R49H) in the BTB domain
180383W118-1_hZBTB7B_D35N_R49Qlentiviral transduction of human ZBTB7B gene with point mutations (D35N and R49Q) in the BTB domain
180384W118-1_hRSK3(RPS6KA2)lentiviral transduction of human RSK3 (RPS6KA2) gene
180385W118-1_hRSK3(RPS6KA2)_K91A_K444Alentiviral transduction of human RSK3 (RPS6KA2) gene with point mutations (K91A and K444A) that inactivate kinase activity
180386AAV.CBA.YFP.miR-E_shControlProducing AAV that encodes negative control shRNA with miR-E backbone
180387AAV.CBA.YFP.miR-E_shAtxn1Producing AAV that encodes mouse Atxn1 shRNA with miR-E backbone
180388AAV.CBA.YFP.miR-E_shRps6ka2-1Producing AAV that encodes mouse Rps6ka2 (Rsk3) shRNA-1 with miR-E backbone
180389AAV.CBA.YFP.miR-E_shRps6ka2-2Producing AAV that encodes mouse Rps6ka2 (Rsk3) shRNA-2 with miR-E backbone
180390AAV.CBA.YFP.miR-E_shRps6ka2-3Producing AAV that encodes mouse Rps6ka2 (Rsk3) shRNA-3 with miR-E backbone

Antibodies from Article