A DREaMR system to simplify combining mutations with rescue transgenes in Aedes aegypti.
Chen J, Luo J, Gurav AS, Chen Z, Wang Y, Montell C
Genetics. 2021 Nov 5;219(3). pii: 6368066. doi: 10.1093/genetics/iyab146.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 182203 | 1xTRE DREaMR 3p3GFP | 1xTRE Tet-On cassette with 3xp3 GFP marker for generating knockins with doxycycline-inducible rescue. |
| 182204 | 1xTRE DREaMR 3p3DsR | 1xTRE Tet-On cassette with 3xp3 DsRed marker for generating knockins with doxycycline-inducible rescue. |
| 182205 | 3xTRE DREaMR 3p3GFP | 3xTRE Tet-On cassette with 3xp3 GFP marker for generating knockins with doxycycline-inducible rescue. |
| 182206 | 3xTRE DREaMR 3p3DsR | 3xTRE Tet-On cassette with 3xp3 DsRed maker for generating knockins with doxycycline-inducible rescue |
| 182207 | DmU6 3p3 GFP LgRNA | For generation of plasmids for CRISPR-mediated 3p3 GFP knockin in Drosophila |
| 182208 | AeU6 3p3 GFP LgRNA | For generation of plasmids for CRISPR-mediated 3p3 GFP knockin in Aedes aegypti |